U2AF1 antibody: A302-079A


Antibody Info
Product_IDA302-079A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB298WVV
Target Info
GeneNameU2AF1
Phosphate
Target_nameU2AF1-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A302-079A_1_U2AF1.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using U2AF1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-U2AF1 antibody (lanes under 'U2AF1').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using U2AF1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-U2AF1 antibody (lanes under 'U2AF1').

Method: immunoprecipitation

Releated Sample: eCLIP:282

Status: Released

Lab: Yeo Lab

U2AF1-HEPG2_Secondary_Western.png<br>Caption: Western blot following shRNA against U2AF1 in HepG2 whole cell lysate using U2AF1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against U2AF1.U2AF1 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against U2AF1 in HepG2 whole cell lysate using U2AF1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against U2AF1.U2AF1 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGHLV30-55

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A302-079A_1_U2AF1.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using U2AF1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-U2AF1 antibody (lanes under 'U2AF1').

Caption: IP-Western Blot analysis of K562 whole cell lysate using U2AF1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-U2AF1 antibody (lanes under 'U2AF1').

Method: immunoprecipitation

Releated Sample: eCLIP:244

Status: Released

Lab: Yeo Lab

U2AF1-k562_Secondary_Western.png<br>Caption: Western blot following shRNA against U2AF1 in K562 whole cell lysate using U2AF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against U2AF1.U2AF1 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against U2AF1 in K562 whole cell lysate using U2AF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against U2AF1.U2AF1 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV08-61

Status: Released

Lab: Graveley Lab