EIF4B antibody: A301-767A


Antibody Info
Product_IDA301-767A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB697PTS
Target Info
GeneNameEIF4B
Phosphate
Target_nameEIF4B-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A301-767A_1_EIF4B.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using EIF4B specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EIF4B antibody (lanes under 'EIF4B').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using EIF4B specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EIF4B antibody (lanes under 'EIF4B').

Method: immunoprecipitation

Releated Sample: eCLIP:626

Status: Released

Lab: Yeo Lab

EIF4B-HEPG2-CRISPR.png<br>Caption: Western blot following CRISPR against EIF4B in HepG2 whole cell lysate using EIF4B specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF4B.EIF4B protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following CRISPR against EIF4B in HepG2 whole cell lysate using EIF4B specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF4B.EIF4B protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGHcLV10-23

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A301-767A_1_EIF4B.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using EIF4B specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EIF4B antibody (lanes under 'EIF4B').

Caption: IP-Western Blot analysis of K562 whole cell lysate using EIF4B specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EIF4B antibody (lanes under 'EIF4B').

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

EIF4B-k562.png<br>Caption: Western blot following shRNA against EIF4B in K562 whole cell lysate using EIF4B specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against EIF4B. EIF4B protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.

Caption: Western blot following shRNA against EIF4B in K562 whole cell lysate using EIF4B specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against EIF4B. EIF4B protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.

Releated Sample: BGKLV08-23

Status: NotSatisfied

Lab: Graveley Lab