CSTF2T antibody: A301-487A


Antibody Info
Product_IDA301-487A
Lot_ID3
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB005DBK
Target Info
GeneNameCSTF2T
Phosphate
Target_nameCSTF2T-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A301-487A_3_CSTF2T.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using CSTF2T specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-CSTF2T antibody (lanes under 'CSTF2T').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using CSTF2T specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-CSTF2T antibody (lanes under 'CSTF2T').

Method: immunoprecipitation

Releated Sample: eCLIP:292

Status: Released

Lab: Yeo Lab

CSTF2T_Secondary_Western.png<br>Caption: Western blot following shRNA against CSTF2T in K562 and HepG2 whole cell lysate using CSTF2T specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against CSTF2T. Lanes 5-7 follow the same pattern, but in HepG2. CSTF2T protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against CSTF2T in K562 and HepG2 whole cell lysate using CSTF2T specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against CSTF2T. Lanes 5-7 follow the same pattern, but in HepG2. CSTF2T protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV13-43

Status: Released

Lab: Graveley Lab

K562 K562_BETHYL_A301487A_1_CSTF2T.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using CSTF2T specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-CSTF2T antibody (lanes under 'CSTF2T').

Caption: IP-Western Blot analysis of K562 whole cell lysate using CSTF2T specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-CSTF2T antibody (lanes under 'CSTF2T').

Method: immunoprecipitation

Releated Sample: eCLIP:275

Status: Released

Lab: Yeo Lab

CSTF2T_Secondary_Western.png<br>Caption: Western blot following shRNA against CSTF2T in K562 and HepG2 whole cell lysate using CSTF2T specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against CSTF2T. Lanes 5-7 follow the same pattern, but in HepG2. CSTF2T protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against CSTF2T in K562 and HepG2 whole cell lysate using CSTF2T specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against CSTF2T. Lanes 5-7 follow the same pattern, but in HepG2. CSTF2T protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV13-43

Status: Released

Lab: Graveley Lab