GEMIN5 antibody: A301-325A


Antibody Info
Product_IDA301-325A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB395KQN
Target Info
GeneNameGEMIN5
Phosphate
Target_nameGEMIN5-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A301-325A_1_GEMIN5.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using GEMIN5 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-GEMIN5 antibody (lanes under 'GEMIN5').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using GEMIN5 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-GEMIN5 antibody (lanes under 'GEMIN5').

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

gemin5_Secondary_Western.png<br>Caption: Western blot following shRNA against FUS in K562 and HepG2 whole cell lysate using gemin-5 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against gemin-5. Lanes 5-7 follow the same pattern, but in HepG2. gemin-5 protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against FUS in K562 and HepG2 whole cell lysate using gemin-5 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against gemin-5. Lanes 5-7 follow the same pattern, but in HepG2. gemin-5 protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGHLV16-29

Status: Released

Lab: Graveley Lab

gemin5_Secondary_Western.png<br>Caption: Western blot following shRNA against FUS in K562 and HepG2 whole cell lysate using gemin-5 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against gemin-5. Lanes 5-7 follow the same pattern, but in HepG2. gemin-5 protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against FUS in K562 and HepG2 whole cell lysate using gemin-5 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against gemin-5. Lanes 5-7 follow the same pattern, but in HepG2. gemin-5 protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGHLV16-29

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A301-325A_1_GEMIN5.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using GEMIN5 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-GEMIN5 antibody (lanes under 'GEMIN5').

Caption: IP-Western Blot analysis of K562 whole cell lysate using GEMIN5 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-GEMIN5 antibody (lanes under 'GEMIN5').

Method: immunoprecipitation

Releated Sample: eCLIP:677

Status: Released

Lab: Yeo Lab