HepG2 | | ![AKAP8-HEPG2_Secondary_Western.png<br>Caption: Western blot following shRNA against AKAP8 in HepG2 whole cell lysate using AKAP8 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against AKAP8.AKAP8 protein appears as the green band, Tubulin serves as a control and appears in red.](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot/AKAP8-HEPG2_Secondary_Western.png)
Caption: Western blot following shRNA against AKAP8 in HepG2 whole cell lysate using AKAP8 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against AKAP8.AKAP8 protein appears as the green band, Tubulin serves as a control and appears in red. Releated Sample: BGHLV27-5 Status: Released Lab: Graveley Lab |
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K562 | ![K562_Bethyl_A301-061A_1_AKAP8.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using AKAP8 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-AKAP8 antibody (lanes under 'AKAP8').](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot_primary/K562_Bethyl_A301-061A_1_AKAP8.png)
Caption: IP-Western Blot analysis of K562 whole cell lysate using AKAP8 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-AKAP8 antibody (lanes under 'AKAP8'). Method: immunoprecipitation Status: Released Lab: Yeo Lab | ![AKAP8-K562.png<br>Caption: Western blot following shRNA against AKAP8 in K562 whole cell lysate using AKAP8 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against AKAP8.AKAP8 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot/AKAP8-K562.png)
Caption: Western blot following shRNA against AKAP8 in K562 whole cell lysate using AKAP8 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against AKAP8.AKAP8 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. Releated Sample: BGKLV32-7 Status: Released Lab: Graveley Lab |
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