AKAP8 antibody: A301-061A


Antibody Info
Product_IDA301-061A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB124CAD
Target Info
GeneNameAKAP8
Phosphate
Target_nameAKAP8-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 AKAP8-HEPG2_Secondary_Western.png<br>Caption: Western blot following shRNA against AKAP8 in HepG2 whole cell lysate using AKAP8 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against AKAP8.AKAP8 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against AKAP8 in HepG2 whole cell lysate using AKAP8 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against AKAP8.AKAP8 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGHLV27-5

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A301-061A_1_AKAP8.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using AKAP8 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-AKAP8 antibody (lanes under 'AKAP8').

Caption: IP-Western Blot analysis of K562 whole cell lysate using AKAP8 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-AKAP8 antibody (lanes under 'AKAP8').

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

AKAP8-K562.png<br>Caption: Western blot following shRNA against AKAP8 in K562 whole cell lysate using AKAP8 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against AKAP8.AKAP8 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against AKAP8 in K562 whole cell lysate using AKAP8 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against AKAP8.AKAP8 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGKLV32-7

Status: Released

Lab: Graveley Lab