Antibodies of AKAP8


2 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
AKAP8Product_ID: A301-062A
Lot_ID: 1
Source: Bethyl Labs
Target Name: AKAP8-human		 Bethyl_A301-062A_1_AKAP8.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the AKAP8 specific antibody, A301-062A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the AKAP8-specific antibody, A301-062A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
AKAP8Product_ID: A301-061A
Lot_ID: 1
Source: Bethyl Labs
Target Name: AKAP8-human		 AKAP8-HEPG2_Secondary_Western.png<br>Caption: Western blot following shRNA against AKAP8 in HepG2 whole cell lysate using AKAP8 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against AKAP8.AKAP8 protein appears as the green band, Tubulin serves as a control and appears in red.
 K562_Bethyl_A301-061A_1_AKAP8.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using AKAP8 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-AKAP8 antibody (lanes under 'AKAP8').
 AKAP8-K562.png<br>Caption: Western blot following shRNA against AKAP8 in K562 whole cell lysate using AKAP8 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against AKAP8.AKAP8 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.