RBFOX2 antibody: A300-864A


Antibody Info
Product_IDA300-864A
Lot_ID2
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB592TEY
Target Info
GeneNameRBFOX2
Phosphate
Target_nameRBFOX2-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A300-864A_2_RBFOX2.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using IGF2BP1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input, lane 2 is 1% of supernatant after IP and lane 3 is 10% of IP enrichment using rabbit polyclonal anti-RBFOX2 antibody (lanes under 'RBFOX2'). Lanes 4, 5 and 6 are in the same pattern but using rabbit normal IgG for IP (lanes under 'IgG').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using IGF2BP1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input, lane 2 is 1% of supernatant after IP and lane 3 is 10% of IP enrichment using rabbit polyclonal anti-RBFOX2 antibody (lanes under 'RBFOX2'). Lanes 4, 5 and 6 are in the same pattern but using rabbit normal IgG for IP (lanes under 'IgG').

Method: immunoprecipitation

Releated Sample: eCLIP:204

Status: Released

Lab: Yeo Lab

RBFOX2_H_Secondary_Western.png<br>Caption: Western blot following shRNA against RBFOX2 in HepG2 whole cell lysate using RBFOX2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against RBFOX2. RBFOX2 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against RBFOX2 in HepG2 whole cell lysate using RBFOX2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against RBFOX2. RBFOX2 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGHLV26-35

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A300-864A_2_RBFOX2.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using RBFOX2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-RBFOX2 antibody (lanes under 'RBFOX2').

Caption: IP-Western Blot analysis of K562 whole cell lysate using RBFOX2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-RBFOX2 antibody (lanes under 'RBFOX2').

Method: immunoprecipitation

Releated Sample: eCLIP:676

Status: Released

Lab: Yeo Lab

RBFOX2_K_Secondary_Western.png<br>Caption: Western blot following shRNA against RBFOX2 in K562 whole cell lysate using RBFOX2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against RBFOX2. RBFOX2 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against RBFOX2 in K562 whole cell lysate using RBFOX2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against RBFOX2. RBFOX2 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV24-47

Status: Released

Lab: Graveley Lab