| HepG2 | | 
Caption: Western blot following shRNA against UBE2L3 in K562 and HepG2 whole cell lysate using UBE2L3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against UBE2L3. Lanes 5-8 follow the same pattern, but in HepG2. UBE2L3 protein appears as the green band, Tubulin serves as a control and appears in red. Releated Sample: BGKLV25-61 Status: Released Lab: Graveley Lab |
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| K562 | 
Caption: IP-WB analysis of K562 whole cell lysate using the UBCH7 specific antibody, A300-737A. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the UBCH7-specific antibody, A300-737A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used. Method: immunoprecipitation Status: Released Lab: Yeo Lab |
Caption: Western blot following shRNA against UBE2L3 in K562 and HepG2 whole cell lysate using UBE2L3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against UBE2L3. Lanes 5-8 follow the same pattern, but in HepG2. UBE2L3 protein appears as the green band, Tubulin serves as a control and appears in red. Releated Sample: BGKLV25-61 Status: Released Lab: Graveley Lab |
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