Antibodies of UBE2L3


2 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
UBE2L3Product_ID: A300-737A
Lot_ID: 1
Source: Bethyl Labs
Target Name: UBE2L3-human		 UBE2L3_Secondary_Western.png<br>Caption: Western blot following shRNA against UBE2L3 in K562 and HepG2 whole cell lysate using UBE2L3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against UBE2L3. Lanes 5-8 follow the same pattern, but in HepG2. UBE2L3 protein appears as the green band, Tubulin serves as a control and appears in red.
 Bethyl_A300-737A_1_UBCH7.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the UBCH7 specific antibody, A300-737A. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the UBCH7-specific antibody, A300-737A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 UBE2L3_Secondary_Western.png<br>Caption: Western blot following shRNA against UBE2L3 in K562 and HepG2 whole cell lysate using UBE2L3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against UBE2L3. Lanes 5-8 follow the same pattern, but in HepG2. UBE2L3 protein appears as the green band, Tubulin serves as a control and appears in red.
UBE2L3Product_ID: 8721S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: UBE2L3-human		 CST_8721S_1_UBE2L3.png<br>Caption: IP-WB analysis of 8721S whole cell lysate using the UBE2L3 specific antibody, 8721S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the UBE2L3-specific antibody, 8721S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 UBE2L3-K562-8721S.png<br>Caption: Western blot following shRNA against UBE2L3 in K562 whole cell lysate using UBE2L3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against UBE2L3. UBE2L3 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.