DDX3X antibody: A300-474A


Antibody Info
Product_IDA300-474A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB173JTT
Target Info
GeneNameDDX3X
Phosphate
Target_nameDDX3X-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A300-474A_1_DDX3X.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DDX3X specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX3X antibody (lanes under 'DDX3X').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DDX3X specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX3X antibody (lanes under 'DDX3X').

Method: immunoprecipitation

Releated Sample: eCLIP:492

Status: Released

Lab: Yeo Lab

DDX3X-HEPG2.png<br>Caption: Western blot following shRNA against DDX3X in HepG2 whole cell lysate using DDX3X specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX3X. DDX3X protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against DDX3X in HepG2 whole cell lysate using DDX3X specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX3X. DDX3X protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGHLV12-15

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A300-474A_1_DDX3X.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using DDX3X specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX3X antibody (lanes under 'DDX3X').

Caption: IP-Western Blot analysis of K562 whole cell lysate using DDX3X specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX3X antibody (lanes under 'DDX3X').

Method: immunoprecipitation

Releated Sample: eCLIP:350

Status: Released

Lab: Yeo Lab

DDX3X_Secondary_Western.png<br>Caption: Western blot following shRNA against DDX3X in K562 whole cell lysate using DDX3X specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX3X. DDX3X protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against DDX3X in K562 whole cell lysate using DDX3X specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX3X. DDX3X protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV08-15

Status: Released

Lab: Graveley Lab