ENCORE Antibody

Product_ID	8588S
Lot_ID	1
Host_organism	Rabbit
Source	Cell Signaling Technology
Antibody_DCC_ID

GeneName	SYNCRIP
Phosphate
Target_name	SYNCRIP-human

Product_ID	8588S
Lot_ID	1
Host_organism	Rabbit
Source	Cell Signaling Technology
Antibody_DCC_ID

GeneName	HNRNPR
Phosphate
Target_name	HNRNPR-human

Validation Images

Cell line	Primary validation	Secondary validation
K562	Caption: IP-WB analysis of 8588S whole cell lysate using the HNRNPQ/HNRNPR specific antibody, 8588S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the HNRNPQ/HNRNPR-specific antibody, 8588S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. NOTE Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used. Method: immunoprecipitation Status: Submitted Lab: Yeo Lab

Cell line

Primary validation

Secondary validation

K562

CST_8588S_1_HNRNPQ.png<br>Caption: IP-WB analysis of 8588S whole cell lysate using the HNRNPQ/HNRNPR specific antibody, 8588S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the HNRNPQ/HNRNPR-specific antibody, 8588S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Caption: IP-WB analysis of 8588S whole cell lysate using the HNRNPQ/HNRNPR specific antibody, 8588S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the HNRNPQ/HNRNPR-specific antibody, 8588S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Method: immunoprecipitation

Status: Submitted

Lab: Yeo Lab

Cell line	Primary validation	Secondary validation
K562	Caption: IP-WB analysis of 8588S whole cell lysate using the HNRNPQ/HNRNPR specific antibody, 8588S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the HNRNPQ/HNRNPR-specific antibody, 8588S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. NOTE Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used. Method: immunoprecipitation Status: Submitted Lab: Yeo Lab