ENCORE Antibody

Product_ID	14634S
Lot_ID	1
Host_organism	Rabbit
Source	Cell Signaling Technology
Antibody_DCC_ID

GeneName	DIAPH1
Phosphate
Target_name	DIAPH1-human

Product_ID	14634S
Lot_ID	2
Host_organism	rabbit
Source	Cell Signaling
Antibody_DCC_ID

GeneName	DIAPH1
Phosphate
Target_name	DIAPH1-human

Validation Images

Cell line	Primary validation	Secondary validation
K562	Caption: IP-WB analysis of 14634S whole cell lysate using the DIAPH1 specific antibody, 14634S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the DIAPH1-specific antibody, 14634S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. NOTE Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used. Method: immunoprecipitation Status: Submitted Lab: Yeo Lab

Cell line

Primary validation

Secondary validation

K562

CST_14634S_1_DIAPH1.png<br>Caption: IP-WB analysis of 14634S whole cell lysate using the DIAPH1 specific antibody, 14634S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the DIAPH1-specific antibody, 14634S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Caption: IP-WB analysis of 14634S whole cell lysate using the DIAPH1 specific antibody, 14634S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the DIAPH1-specific antibody, 14634S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Method: immunoprecipitation

Status: Submitted

Lab: Yeo Lab

Cell line	Primary validation	Secondary validation
K562		Caption: Western blot following CRISPR against DIAPH1 in K562 whole cell lysate using DIAPH1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DIAPH1. DIAPH1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow. Releated Sample: BGKcLV38-11 Status: Submitted Lab: Graveley Lab

Cell line

Primary validation

Secondary validation

K562

Caption: Western blot following CRISPR against DIAPH1 in K562 whole cell lysate using DIAPH1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DIAPH1. DIAPH1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Releated Sample: BGKcLV38-11

Status: Submitted

Lab: Graveley Lab

DIAPH1 antibody: 14634S