TRIM55 RNA-Seq in Ribo-STAMP-293T

Experiment Information (Status: Submitted)

	BGRSDLV04-97	BGRSDLV04-98
idx	0	0
TRCN#_or_BGC#	TRCN0000034009	TRCN0000034009
shRNA_or_gRNA_sequence	CCGGGCTACTTCTCAGGAGTTAGTACTCGAGTACTAACTCCTGAGAAGTAGCTTTTTG	CCGGGCTACTTCTCAGGAGTTAGTACTCGAGTACTAACTCCTGAGAAGTAGCTTTTTG
PAM
Name	TRIM55	TRIM55
Sample_ID	BGRSDLV04-97	BGRSDLV04-98
transduction_Date	10/16/23	10/16/23
days	4 days	4 days
RBP_name	TRIM55	TRIM55
qPCR_result
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc	0	0
WB_result
Ave_WB
WB_DONE_date
MW
IP
antibody_Cat#
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note
ID	9461	9462

Western Blot Western Blot info was not avaliable

Experiment Status

	BGRSDLV04-97	BGRSDLV04-98
Sample_ID	BGRSDLV04-97	BGRSDLV04-98
Sample Name
Sample Name	TRIM55-BGRSDLV04-97	TRIM55-BGRSDLV04-98
RBP	TRIM55	TRIM55
Cell_Line	DOX-RS293	DOX-RS293
Exp UID
Status	Submitted	Submitted
Status_date	2024-12-03	2024-12-03
Project	ENCORE2	ENCORE2
Note
ID	12698	12699

Library-Prep
Sequencing

Library-Prep Information

	BGRSDLV04-97	BGRSDLV04-98
Sample #	53	54
Sample Name	TRIM55-BGRSDLV04-97	TRIM55-BGRSDLV04-98
Sample_Name_Alias
Index Well Position	E07	F07
Index_table	IDT_UniqueDualIndex_96	IDT_UniqueDualIndex_96
LibPrep_date	2023-11-14	2023-11-14
Lib_ID	Lib-231114	Lib-231114
Tecan_Location
Tecan
Tecan_date
Size_bp	288	289
Peak_Molarity	40.00	68.80
libSampleQC_DNA_Well	DNA_Library_set68/set1/E7.png	DNA_Library_set68/set1/F7.png
RIN	10.0	10.0
libSample_RNA_Well	RNA_Library_set68/set1/E7.png	RNA_Library_set68/set1/F7.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2023-11-16	2023-11-16
Sample_ID	BGRSDLV04-97	BGRSDLV04-98
RBP	TRIM55	TRIM55
Batch_ID	BGRSDLV04	BGRSDLV04
WB_result	0.000	0.000
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	4126	4127

Sequencing Information

	BGRSDLV04-97	BGRSDLV04-98
Sample_ID	BGRSDLV04-97	BGRSDLV04-98
Sample Name	TRIM55-BGRSDLV04-97	TRIM55-BGRSDLV04-98
Pool ID	Pool-231116	Pool-231116
LocalServer_folder	set68	set68
total_reads	39,499,718	61,360,287
total_aligned_reads	31,441,203	56,760,323
unique_aligned_reads	29,517,764	53,470,688
percent_uniqueAligned	0.74729	0.87142
correlation_replicates	0.996858	0.996858
spikein_reads	6,556	10,166
percent_spikeins	0.00017	0.00017
original_ReadLength	151	151
QC_Status	Submitted	Submitted
ID	2889	2890

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
TRIM55-BGRSDLV04-97_S53_L004_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set68/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	2889	NovaSeq6000	100	paired-ended	1				NT-BGRSDLV04-1_S1_L004_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGRSDLV04-2_S2_L004_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	27666
TRIM55-BGRSDLV04-97_S53_L004_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set68/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	2889	NovaSeq6000	100	paired-ended	1				NT-BGRSDLV04-1_S1_L004_R2_001.filtered.trimmed.paired.fastq.gz,NT-BGRSDLV04-2_S2_L004_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	27730
TRIM55-BGRSDLV04-97_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set68/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	2889					GRCh38	V40	TRIM55-BGRSDLV04-97_S53_L004_R1_001.filtered.trimmed.paired.fastq.gz,TRIM55-BGRSDLV04-97_S53_L004_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	27794
TRIM55-BGRSDLV04-97_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set68/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	2889					GRCh38	V40	TRIM55-BGRSDLV04-97/Aligned.sortedByCoord.out.bam		ENCORE2	27858
TRIM55-BGRSDLV04-97_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set68/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	2889					GRCh38	V40	TRIM55-BGRSDLV04-97/Aligned.sortedByCoord.out.bam		ENCORE2	27922
TRIM55-BGRSDLV04-97_Signal.Unique.strand-.bw	ENCODE_DATA/set68/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	2889					GRCh38	V40	TRIM55-BGRSDLV04-97/Aligned.sortedByCoord.out.bam		ENCORE2	27986
TRIM55-BGRSDLV04-97_Signal.Unique.strand+.bw	ENCODE_DATA/set68/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	2889					GRCh38	V40	TRIM55-BGRSDLV04-97/Aligned.sortedByCoord.out.bam		ENCORE2	28050
TRIM55-BGRSDLV04-97_quant.sf	ENCODE_DATA/set68/salmon_ENCORE2	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	2889					GRCh38	V40	TRIM55-BGRSDLV04-97_S53_L004_R1_001.filtered.trimmed.paired.fastq.gz,TRIM55-BGRSDLV04-97_S53_L004_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	28114
TRIM55-BGRSDLV04-98_S54_L004_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set68/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	2890	NovaSeq6000	100	paired-ended	1				NT-BGRSDLV04-1_S1_L004_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGRSDLV04-2_S2_L004_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	27667
TRIM55-BGRSDLV04-98_S54_L004_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set68/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	2890	NovaSeq6000	100	paired-ended	1				NT-BGRSDLV04-1_S1_L004_R2_001.filtered.trimmed.paired.fastq.gz,NT-BGRSDLV04-2_S2_L004_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	27731
TRIM55-BGRSDLV04-98_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set68/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	2890					GRCh38	V40	TRIM55-BGRSDLV04-98_S54_L004_R1_001.filtered.trimmed.paired.fastq.gz,TRIM55-BGRSDLV04-98_S54_L004_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	27795
TRIM55-BGRSDLV04-98_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set68/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	2890					GRCh38	V40	TRIM55-BGRSDLV04-98/Aligned.sortedByCoord.out.bam		ENCORE2	27859
TRIM55-BGRSDLV04-98_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set68/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	2890					GRCh38	V40	TRIM55-BGRSDLV04-98/Aligned.sortedByCoord.out.bam		ENCORE2	27923
TRIM55-BGRSDLV04-98_Signal.Unique.strand-.bw	ENCODE_DATA/set68/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	2890					GRCh38	V40	TRIM55-BGRSDLV04-98/Aligned.sortedByCoord.out.bam		ENCORE2	27987
TRIM55-BGRSDLV04-98_Signal.Unique.strand+.bw	ENCODE_DATA/set68/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	2890					GRCh38	V40	TRIM55-BGRSDLV04-98/Aligned.sortedByCoord.out.bam		ENCORE2	28051
TRIM55-BGRSDLV04-98_quant.sf	ENCODE_DATA/set68/salmon_ENCORE2	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	2890					GRCh38	V40	TRIM55-BGRSDLV04-98_S54_L004_R1_001.filtered.trimmed.paired.fastq.gz,TRIM55-BGRSDLV04-98_S54_L004_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	28115