CNBP RNA-Seq in Ribo-STAMP-293T

Experiment Information (Status: Submitted)

	BGRSDLV02-17	BGRSDLV02-18
idx	0	0
TRCN#_or_BGC#	TRCN0000218324	TRCN0000218324
shRNA_or_gRNA_sequence	GTACCGGATTGAGGCTACAGCCTAATTACTCGAGTAATTAGGCTGTAGCCTCAATTTTTTTG	GTACCGGATTGAGGCTACAGCCTAATTACTCGAGTAATTAGGCTGTAGCCTCAATTTTTTTG
PAM
Name	CNBP	CNBP
Sample_ID	BGRSDLV02-17	BGRSDLV02-18
transduction_Date	9/15/23	9/15/23
days	4 days	4 days
RBP_name	CNBP	CNBP
qPCR_result	93.5	93.9
Ave_qPCR	93.7
RT-qPCR_primer-F	tatcgctgtggtgagtctgg
RT-qPCR_primer-R	gcattgctctcgctctctct
protein_conc	0	0
WB_result
Ave_WB
WB_DONE_date
MW
IP
antibody_Cat#
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note
ID	9228	9229

Western Blot Western Blot info was not avaliable

Experiment Status

	BGRSDLV02-17	BGRSDLV02-18
Sample_ID	BGRSDLV02-17	BGRSDLV02-18
Sample Name
Sample Name	CNBP-BGRSDLV02-17	CNBP-BGRSDLV02-18
RBP	CNBP	CNBP
Cell_Line	DOX-RS293	DOX-RS293
Exp UID
Status	Submitted	Submitted
Status_date	2024-12-03	2024-12-03
Project	ENCORE2	ENCORE2
Note
ID	12336	12337

Library-Prep
Sequencing

Library-Prep Information

	BGRSDLV02-17	BGRSDLV02-18
Sample #	17	18
Sample Name	CNBP-BGRSDLV02-17	CNBP-BGRSDLV02-18
Sample_Name_Alias
Index Well Position	A03	B03
Index_table	IDT_UniqueDualIndex_96	IDT_UniqueDualIndex_96
LibPrep_date	2023-09-26	2023-09-26
Lib_ID	Lib-230926	Lib-230926
Tecan_Location
Tecan
Tecan_date
Size_bp	291	288
Peak_Molarity	100.00	80.10
libSampleQC_DNA_Well	DNA_Library_set65/set1/A3.png	DNA_Library_set65/set1/B3.png
RIN	9.9	10.0
libSample_RNA_Well	RNA_Library_set65/set1/A3.png	RNA_Library_set65/set1/B3.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2023-09-27	2023-09-27
Sample_ID	BGRSDLV02-17	BGRSDLV02-18
RBP	CNBP	CNBP
Batch_ID	BGRSDLV02	BGRSDLV02
WB_result	0.000	0.000
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	3938	3939

Sequencing Information

	BGRSDLV02-17	BGRSDLV02-18
Sample_ID	BGRSDLV02-17	BGRSDLV02-18
Sample Name	CNBP-BGRSDLV02-17	CNBP-BGRSDLV02-18
Pool ID	Pool-231003	Pool-231003
LocalServer_folder	set65	set65
total_reads	23,509,781	23,750,829
total_aligned_reads	20,879,744	21,480,315
unique_aligned_reads	19,536,628	20,073,382
percent_uniqueAligned	0.83100	0.84517
correlation_replicates	0.995489	0.995489
spikein_reads	2,032	640
percent_spikeins	0.00009	0.00003
original_ReadLength	100	100
QC_Status	Submitted	Submitted
ID	2685	2686

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
CNBP-BGRSDLV02-17_S17_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set65/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	2685	NovaSeq6000	100	paired-ended	1				NT-BGRSDLV02-1_S1_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGRSDLV02-2_S2_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	8754
CNBP-BGRSDLV02-17_S17_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set65/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	2685	NovaSeq6000	100	paired-ended	2				NT-BGRSDLV02-1_S1_L001_R2_001.filtered.trimmed.paired.fastq.gz,NT-BGRSDLV02-2_S2_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	8830
CNBP-BGRSDLV02-17_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set65/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	2685					GRCh38	V40	CNBP-BGRSDLV02-17_S17_L001_R1_001.filtered.trimmed.paired.fastq.gz,CNBP-BGRSDLV02-17_S17_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	9319
CNBP-BGRSDLV02-17_Signal.Unique.strand+.bw	ENCODE_DATA/set65/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	2685					GRCh38	V40	CNBP-BGRSDLV02-17/Aligned.sortedByCoord.out.bam		ENCORE2	9395
CNBP-BGRSDLV02-17_Signal.Unique.strand-.bw	ENCODE_DATA/set65/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	2685					GRCh38	V40	CNBP-BGRSDLV02-17/Aligned.sortedByCoord.out.bam		ENCORE2	9471
CNBP-BGRSDLV02-17_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set65/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	2685					GRCh38	V40	CNBP-BGRSDLV02-17/Aligned.sortedByCoord.out.bam		ENCORE2	9547
CNBP-BGRSDLV02-17_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set65/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	2685					GRCh38	V40	CNBP-BGRSDLV02-17/Aligned.sortedByCoord.out.bam		ENCORE2	9623
CNBP-BGRSDLV02-17_quant.sf	ENCODE_DATA/set65/salmon_ENCORE2	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	2685					GRCh38	V40	CNBP-BGRSDLV02-17_S17_L001_R1_001.filtered.trimmed.paired.fastq.gz,CNBP-BGRSDLV02-17_S17_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	9699
CNBP-BGRSDLV02-18_S18_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set65/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	2686	NovaSeq6000	100	paired-ended	1				NT-BGRSDLV02-1_S1_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGRSDLV02-2_S2_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	8755
CNBP-BGRSDLV02-18_S18_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set65/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	2686	NovaSeq6000	100	paired-ended	2				NT-BGRSDLV02-1_S1_L001_R2_001.filtered.trimmed.paired.fastq.gz,NT-BGRSDLV02-2_S2_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	8831
CNBP-BGRSDLV02-18_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set65/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	2686					GRCh38	V40	CNBP-BGRSDLV02-18_S18_L001_R1_001.filtered.trimmed.paired.fastq.gz,CNBP-BGRSDLV02-18_S18_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	9320
CNBP-BGRSDLV02-18_Signal.Unique.strand+.bw	ENCODE_DATA/set65/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	2686					GRCh38	V40	CNBP-BGRSDLV02-18/Aligned.sortedByCoord.out.bam		ENCORE2	9396
CNBP-BGRSDLV02-18_Signal.Unique.strand-.bw	ENCODE_DATA/set65/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	2686					GRCh38	V40	CNBP-BGRSDLV02-18/Aligned.sortedByCoord.out.bam		ENCORE2	9472
CNBP-BGRSDLV02-18_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set65/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	2686					GRCh38	V40	CNBP-BGRSDLV02-18/Aligned.sortedByCoord.out.bam		ENCORE2	9548
CNBP-BGRSDLV02-18_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set65/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	2686					GRCh38	V40	CNBP-BGRSDLV02-18/Aligned.sortedByCoord.out.bam		ENCORE2	9624
CNBP-BGRSDLV02-18_quant.sf	ENCODE_DATA/set65/salmon_ENCORE2	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	2686					GRCh38	V40	CNBP-BGRSDLV02-18_S18_L001_R1_001.filtered.trimmed.paired.fastq.gz,CNBP-BGRSDLV02-18_S18_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	9700