HMGB1      CRISPR in K562      Batch: BGKcLV53

Experiment Information (Status: Sequencing)
BGKcLV53-67BGKcLV53-68
idx00
TRCN#_or_BGC#BGC#0001515BGC#0001515
shRNA_or_gRNA_sequenceTGTGTGTCCTGTGTACCCGATGTGTGTCCTGTGTACCCGA
PAMGGGGGG
NameHMGB1_88UHMGB1_88U
Sample_IDBGKcLV53-67BGKcLV53-68
transduction_Date2/24/262/24/26
daysD6D6
RBP_nameHMGB1HMGB1
qPCR_result0.022.0
Ave_qPCR11.0
RT-qPCR_primer-Ftatggcaaaagcggacaagg
RT-qPCR_primer-Rtttgggcgatactcagagca
protein_conc18811777
WB_result58.859.7
Ave_WB
WB_DONE_date3/24/26,3/20/26
MW25kd#69
IP
antibody_Cat#GTX101277lot# 39742
Antibody DCC IDENCAB976FQA
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID64296430




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
HMGB1Product_ID: GTX101277
Lot_ID: 39742
Source: GeneTex
Target Name: HMGB1-human		 HMGB1-K562-CRISPR-GTX101277.png<br>Caption: Western blot following CRISPR against HMGB1 in K562 whole cell lysate using HMGB1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against HMGB1. HMGB1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV53-67BGKcLV53-68
Sample_IDBGKcLV53-67BGKcLV53-68
Sample Name
Sample NameHMGB1-BGKcLV53-67HMGB1-BGKcLV53-68
RBPHMGB1HMGB1
Cell_LineK562K562
Exp UID
StatusSequencingSequencing
Status_date2026-06-022026-06-02
ProjectENCORE2ENCORE2
Note
ID1479614797




Library-Prep Information
BGKcLV53-67BGKcLV53-68
Sample #4748
Sample NameHMGB1-BGKcLV53-67HMGB1-BGKcLV53-68
Sample_Name_Alias
Index Well PositionE07F07
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2026-05-262026-05-26
Lib_IDLib-260526Lib-260526
Tecan_Location
Tecan
Tecan_date
Size_bp296307
Peak_Molarity85.3066.50
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2026-05-272026-05-27
Sample_IDBGKcLV53-67BGKcLV53-68
RBPHMGB1HMGB1
Batch_IDBGKcLV53BGKcLV53
WB_result58.80059.700
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID48864887




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database