RPL18 RNA-Seq in K562

Experiment Information (Status: Sequenced)

	BGKcLV53-103	BGKcLV53-104
idx	0	0
TRCN#_or_BGC#	BGC#0000621	BGC#0000621
shRNA_or_gRNA_sequence	CCTTCCCTAGGTCCTCGCAA	CCTTCCCTAGGTCCTCGCAA
PAM	GGG	GGG
Name	RPL18_88F	RPL18_88F
Sample_ID	BGKcLV53-103	BGKcLV53-104
transduction_Date	2/24/26	2/24/26
days	D6	D6
RBP_name	RPL18	RPL18
qPCR_result	0.0	0.3
Ave_qPCR	0.2
RT-qPCR_primer-F	ggttgtggggaccataactg
RT-qPCR_primer-R	tggtcgaaagtgaggatcttg
protein_conc	2887	2407
WB_result	65.7	50.2021
Ave_WB
WB_DONE_date	3/31/26
MW	22kd
IP
antibody_Cat#	A305-340A	lot #1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note
ID	6465	6466

Western Blot

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
RPL18	Product_ID: A305-340A Lot_ID: 1 Source: Bethyl Labs Target Name: RPL18-human

Experiment Status

	BGKcLV53-103	BGKcLV53-104
Sample_ID	BGKcLV53-103	BGKcLV53-104
Sample Name
Sample Name	RPL18-BGKcLV53-103	RPL18-BGKcLV53-104
RBP	RPL18	RPL18
Cell_Line	K562	K562
Exp UID
Status	Sequenced	Sequenced
Status_date	2026-09-16	2026-09-16
Project	ENCORE2	ENCORE2
Note
ID	14802	14803

Library-Prep
Sequencing

Library-Prep Information

	BGKcLV53-103	BGKcLV53-104
Sample #	53	54
Sample Name	RPL18-BGKcLV53-103	RPL18-BGKcLV53-104
Sample_Name_Alias
Index Well Position	C08	D08
Index_table	IDT_UniqueDualIndex_96_V2	IDT_UniqueDualIndex_96_V2
LibPrep_date	2026-05-26	2026-05-26
Lib_ID	Lib-260526	Lib-260526
Tecan_Location
Tecan
Tecan_date
Size_bp	282	286
Peak_Molarity	68.70	73.20
libSampleQC_DNA_Well	DNA_Library_set82/set2/C11.png	DNA_Library_set82/set2/D11.png
RIN	10.0	10.0
libSample_RNA_Well	RNA_Library_set82/set2/C8.png	RNA_Library_set82/set2/D8.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2026-05-27	2026-05-27
Sample_ID	BGKcLV53-103	BGKcLV53-104
RBP	RPL18	RPL18
Batch_ID	BGKcLV53	BGKcLV53
WB_result	65.700	50.202
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	4892	4893

Sequencing Information

	BGKcLV53-103	BGKcLV53-104
Sample_ID	BGKcLV53-103	BGKcLV53-104
Sample Name	RPL18-BGKcLV53-103	RPL18-BGKcLV53-104
Pool ID	Pool-260528	Pool-260528
LocalServer_folder	set82	set82
total_reads	31,893,004	32,078,874
total_aligned_reads	24,738,368	28,977,294
unique_aligned_reads	22,867,715	26,859,654
percent_uniqueAligned	0.71701	0.83730
correlation_replicates	0.999683	0.999683
spikein_reads	28,202	26,200
percent_spikeins	0.00088	0.00082
original_ReadLength	151	151
QC_Status	ReadyToSubmit	ReadyToSubmit
ID	3593	3594

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
RPL18-BGKcLV53-103_R1.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set82/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3593	NovaSeq6000	100	paired-ended	1				NT-BGKcLV53-1_R1.filtered.trimmed.paired.fastq.gz,NT-BGKcLV53-2_R1.filtered.trimmed.paired.fastq.gz	ENCORE2	33688
RPL18-BGKcLV53-103_R2.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set82/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3593	NovaSeq6000	100	paired-ended	2				NT-BGKcLV53-1_R2.filtered.trimmed.paired.fastq.gz,NT-BGKcLV53-2_R2.filtered.trimmed.paired.fastq.gz	ENCORE2	33746
RPL18-BGKcLV53-103_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3593					GRCh38	V40	RPL18-BGKcLV53-103_R1.filtered.trimmed.paired.fastq.gz,RPL18-BGKcLV53-103_R2.filtered.trimmed.paired.fastq.gz		ENCORE2	33806
RPL18-BGKcLV53-103_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3593					GRCh38	V40	RPL18-BGKcLV53-103_Aligned.sortedByCoord.out.bam		ENCORE2	33864
RPL18-BGKcLV53-103_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3593					GRCh38	V40	RPL18-BGKcLV53-103_Aligned.sortedByCoord.out.bam		ENCORE2	33922
RPL18-BGKcLV53-103_Signal.Unique.strand-.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3593					GRCh38	V40	RPL18-BGKcLV53-103_Aligned.sortedByCoord.out.bam		ENCORE2	33980
RPL18-BGKcLV53-103_Signal.Unique.strand+.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3593					GRCh38	V40	RPL18-BGKcLV53-103_Aligned.sortedByCoord.out.bam		ENCORE2	34038
RPL18-BGKcLV53-103_quant.sf	ENCODE_DATA/set82/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3593					GRCh38	V40	RPL18-BGKcLV53-103_R1.filtered.trimmed.paired.fastq.gz,RPL18-BGKcLV53-103_R2.filtered.trimmed.paired.fastq.gz		ENCORE2	34096
RPL18-BGKcLV53-104_R1.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set82/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3594	NovaSeq6000	100	paired-ended	1				NT-BGKcLV53-1_R1.filtered.trimmed.paired.fastq.gz,NT-BGKcLV53-2_R1.filtered.trimmed.paired.fastq.gz	ENCORE2	33689
RPL18-BGKcLV53-104_R2.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set82/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3594	NovaSeq6000	100	paired-ended	2				NT-BGKcLV53-1_R2.filtered.trimmed.paired.fastq.gz,NT-BGKcLV53-2_R2.filtered.trimmed.paired.fastq.gz	ENCORE2	33747
RPL18-BGKcLV53-104_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3594					GRCh38	V40	RPL18-BGKcLV53-104_R1.filtered.trimmed.paired.fastq.gz,RPL18-BGKcLV53-104_R2.filtered.trimmed.paired.fastq.gz		ENCORE2	33807
RPL18-BGKcLV53-104_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3594					GRCh38	V40	RPL18-BGKcLV53-104_Aligned.sortedByCoord.out.bam		ENCORE2	33865
RPL18-BGKcLV53-104_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3594					GRCh38	V40	RPL18-BGKcLV53-104_Aligned.sortedByCoord.out.bam		ENCORE2	33923
RPL18-BGKcLV53-104_Signal.Unique.strand-.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3594					GRCh38	V40	RPL18-BGKcLV53-104_Aligned.sortedByCoord.out.bam		ENCORE2	33981
RPL18-BGKcLV53-104_Signal.Unique.strand+.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3594					GRCh38	V40	RPL18-BGKcLV53-104_Aligned.sortedByCoord.out.bam		ENCORE2	34039
RPL18-BGKcLV53-104_quant.sf	ENCODE_DATA/set82/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3594					GRCh38	V40	RPL18-BGKcLV53-104_R1.filtered.trimmed.paired.fastq.gz,RPL18-BGKcLV53-104_R2.filtered.trimmed.paired.fastq.gz		ENCORE2	34097