RC3H1      CRISPR in K562      Batch: BGKcLV51

Experiment Information (Status: Sequencing)
BGKcLV51-85BGKcLV51-86
idx00
TRCN#_or_BGC#BGC#0001479BGC#0001479
shRNA_or_gRNA_sequenceAGGCAGGATTCGTGCCATGAAGGCAGGATTCGTGCCATGA
PAMGGGGGG
NameRC3H1_79BRC3H1_79B
Sample_IDBGKcLV51-85BGKcLV51-86
transduction_Date9/30/20259/30/2025
daysD6D6
RBP_nameRC3H1RC3H1
qPCR_result0.014.0
Ave_qPCR0
RT-qPCR_primer-Ftatccagtggctcctcatcc
RT-qPCR_primer-Ragctgggccattatttcctt
protein_conc21842200
WB_result67.564.7
Ave_WB
WB_DONE_date1/23/26
MW126kd
IP
antibody_Cat#A300-515Alot# 2
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID63266327




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RC3H1Product_ID: A300-515A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RC3H1-human		 Bethyl_A300-515A_1_RC3H1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RC3H1 specific antibody, A300-515A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RC3H1-specific antibody, A300-515A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 RC3H1-K562-CRISPR-A300-515A.png<br>Caption: Western blot following CRISPR against RC3H1 in K562 whole cell lysate using RC3H1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RC3H1. RC3H1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV51-85BGKcLV51-86
Sample_IDBGKcLV51-85BGKcLV51-86
Sample Name
Sample NameRC3H1-BGKcLV51-85RC3H1-BGKcLV51-86
RBPRC3H1RC3H1
Cell_LineK562K562
Exp UID
StatusSequencingSequencing
Status_date2026-06-022026-06-02
ProjectENCORE2ENCORE2
Note
ID1458014581




Library-Prep Information
BGKcLV51-85BGKcLV51-86
Sample #1516
Sample NameRC3H1-BGKcLV51-85RC3H1-BGKcLV51-86
Sample_Name_Alias
Index Well PositionG02H02
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2026-05-182026-05-18
Lib_IDLib-260518Lib-260518
Tecan_Location
Tecan
Tecan_date
Size_bp284282
Peak_Molarity50.6041.80
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2026-05-212026-05-21
Sample_IDBGKcLV51-85BGKcLV51-86
RBPRC3H1RC3H1
Batch_IDBGKcLV51BGKcLV51
WB_result67.50064.700
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID48544855




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database