AGO2 RNA-Seq in K562

Experiment Information (Status: Sequenced)

	BGKcLV51-41	BGKcLV51-42
idx	0	0
TRCN#_or_BGC#	BGC#0001462	BGC#0001462
shRNA_or_gRNA_sequence	GGGGCCGGCTCCCGAGTACA	GGGGCCGGCTCCCGAGTACA
PAM	TGG	TGG
Name	EIF2C2_85	EIF2C2_85
Sample_ID	BGKcLV51-41	BGKcLV51-42
transduction_Date	9/30/2025	9/30/2025
days	D6	D6
RBP_name	AGO2	AGO2
qPCR_result	47.6	56.7
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc	2413	2565
WB_result	72.0	81.7
Ave_WB
WB_DONE_date	2/5/26
MW	97kd
IP
antibody_Cat#	MA5-14861
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note
ID	6282	6283

Western Blot Western Blot info was not avaliable

Experiment Status

	BGKcLV51-41	BGKcLV51-42
Sample_ID	BGKcLV51-41	BGKcLV51-42
Sample Name
Sample Name	AGO2-BGKcLV51-41	AGO2-BGKcLV51-42
RBP	AGO2	AGO2
Cell_Line	K562	K562
Exp UID
Status	Sequenced	Sequenced
Status_date	2026-09-16	2026-09-16
Project	ENCORE2	ENCORE2
Note
ID	14574	14575

Library-Prep
Sequencing

Library-Prep Information

	BGKcLV51-41	BGKcLV51-42
Sample #	9	10
Sample Name	AGO2-BGKcLV51-41	AGO2-BGKcLV51-42
Sample_Name_Alias
Index Well Position	A02	B02
Index_table	IDT_UniqueDualIndex_96_V2	IDT_UniqueDualIndex_96_V2
LibPrep_date	2026-05-18	2026-05-18
Lib_ID	Lib-260518	Lib-260518
Tecan_Location
Tecan
Tecan_date
Size_bp	287	299
Peak_Molarity	42.30	41.30
libSampleQC_DNA_Well	DNA_Library_set82/set1/A9.png	DNA_Library_set82/set1/B9.png
RIN	9.9	9.9
libSample_RNA_Well	RNA_Library_set82/set1/A2.png	RNA_Library_set82/set1/B2.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2026-05-21	2026-05-21
Sample_ID	BGKcLV51-41	BGKcLV51-42
RBP	AGO2	AGO2
Batch_ID	BGKcLV51	BGKcLV51
WB_result	72.000	81.700
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	4848	4849

Sequencing Information

	BGKcLV51-41	BGKcLV51-42
Sample_ID	BGKcLV51-41	BGKcLV51-42
Sample Name	AGO2-BGKcLV51-41	AGO2-BGKcLV51-42
Pool ID	Pool-260528	Pool-260528
LocalServer_folder	set82	set82
total_reads	24,322,758	31,358,206
total_aligned_reads	21,076,074	26,829,612
unique_aligned_reads	19,632,884	25,123,121
percent_uniqueAligned	0.80718	0.80117
correlation_replicates	0.987426	0.987426
spikein_reads	96,274	113,056
percent_spikeins	0.00396	0.00361
original_ReadLength	151	151
QC_Status	ReadyToSubmit	ReadyToSubmit
ID	3549	3550

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
AGO2-BGKcLV51-41_R1.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set82/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3549	NovaSeq6000	100	paired-ended	1				NT-BGKcLV51-1_R1.filtered.trimmed.paired.fastq.gz,NT-BGKcLV51-2_R1.filtered.trimmed.paired.fastq.gz	ENCORE2	33652
AGO2-BGKcLV51-41_R2.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set82/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3549	NovaSeq6000	100	paired-ended	2				NT-BGKcLV51-1_R2.filtered.trimmed.paired.fastq.gz,NT-BGKcLV51-2_R2.filtered.trimmed.paired.fastq.gz	ENCORE2	33710
AGO2-BGKcLV51-41_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3549					GRCh38	V40	AGO2-BGKcLV51-41_R1.filtered.trimmed.paired.fastq.gz,AGO2-BGKcLV51-41_R2.filtered.trimmed.paired.fastq.gz		ENCORE2	33768
AGO2-BGKcLV51-41_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3549					GRCh38	V40	AGO2-BGKcLV51-41_Aligned.sortedByCoord.out.bam		ENCORE2	33826
AGO2-BGKcLV51-41_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3549					GRCh38	V40	AGO2-BGKcLV51-41_Aligned.sortedByCoord.out.bam		ENCORE2	33884
AGO2-BGKcLV51-41_Signal.Unique.strand-.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3549					GRCh38	V40	AGO2-BGKcLV51-41_Aligned.sortedByCoord.out.bam		ENCORE2	33942
AGO2-BGKcLV51-41_Signal.Unique.strand+.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3549					GRCh38	V40	AGO2-BGKcLV51-41_Aligned.sortedByCoord.out.bam		ENCORE2	34000
AGO2-BGKcLV51-41_quant.sf	ENCODE_DATA/set82/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3549					GRCh38	V40	AGO2-BGKcLV51-41_R1.filtered.trimmed.paired.fastq.gz,AGO2-BGKcLV51-41_R2.filtered.trimmed.paired.fastq.gz		ENCORE2	34058
AGO2-BGKcLV51-42_R1.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set82/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3550	NovaSeq6000	100	paired-ended	1				NT-BGKcLV51-1_R1.filtered.trimmed.paired.fastq.gz,NT-BGKcLV51-2_R1.filtered.trimmed.paired.fastq.gz	ENCORE2	33653
AGO2-BGKcLV51-42_R2.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set82/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3550	NovaSeq6000	100	paired-ended	2				NT-BGKcLV51-1_R2.filtered.trimmed.paired.fastq.gz,NT-BGKcLV51-2_R2.filtered.trimmed.paired.fastq.gz	ENCORE2	33711
AGO2-BGKcLV51-42_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3550					GRCh38	V40	AGO2-BGKcLV51-42_R1.filtered.trimmed.paired.fastq.gz,AGO2-BGKcLV51-42_R2.filtered.trimmed.paired.fastq.gz		ENCORE2	33769
AGO2-BGKcLV51-42_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3550					GRCh38	V40	AGO2-BGKcLV51-42_Aligned.sortedByCoord.out.bam		ENCORE2	33827
AGO2-BGKcLV51-42_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3550					GRCh38	V40	AGO2-BGKcLV51-42_Aligned.sortedByCoord.out.bam		ENCORE2	33885
AGO2-BGKcLV51-42_Signal.Unique.strand-.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3550					GRCh38	V40	AGO2-BGKcLV51-42_Aligned.sortedByCoord.out.bam		ENCORE2	33943
AGO2-BGKcLV51-42_Signal.Unique.strand+.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3550					GRCh38	V40	AGO2-BGKcLV51-42_Aligned.sortedByCoord.out.bam		ENCORE2	34001
AGO2-BGKcLV51-42_quant.sf	ENCODE_DATA/set82/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3550					GRCh38	V40	AGO2-BGKcLV51-42_R1.filtered.trimmed.paired.fastq.gz,AGO2-BGKcLV51-42_R2.filtered.trimmed.paired.fastq.gz		ENCORE2	34059