SEPT7 RNA-Seq in K562

Experiment Information (Status: Sequenced)

	BGKcLV51-105	BGKcLV51-106
idx	0	0
TRCN#_or_BGC#	BGC#0001484	BGC#0001484
shRNA_or_gRNA_sequence	AAGAACCTTGAAGGCTATGT	AAGAACCTTGAAGGCTATGT
PAM	GGG	GGG
Name	SEPTIN7_63	SEPTIN7_63
Sample_ID	BGKcLV51-105	BGKcLV51-106
transduction_Date	9/30/2025	9/30/2025
days	D6	D6
RBP_name	SEPT7	SEPT7
qPCR_result	0.0	0.0
Ave_qPCR	0.0
RT-qPCR_primer-F	aggatttggagatgcagtgg
RT-qPCR_primer-R	aggcatctgacgtctgttca
protein_conc	1007	1053
WB_result	53.1	56.6
Ave_WB
WB_DONE_date	11/19/25
MW
IP
antibody_Cat#	A304-212A	lot# 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note
ID	6346	6347

Western Blot

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
SEPT7	Product_ID: A304-212A Lot_ID: 1 Source: Bethyl Labs Target Name: SEPT7-human

Experiment Status

	BGKcLV51-105	BGKcLV51-106
Sample_ID	BGKcLV51-105	BGKcLV51-106
Sample Name
Sample Name	SEPT7-BGKcLV51-105	SEPT7-BGKcLV51-106
RBP	SEPT7	SEPT7
Cell_Line	K562	K562
Exp UID
Status	Sequenced	Sequenced
Status_date	2026-09-16	2026-09-16
Project	ENCORE2	ENCORE2
Note
ID	14584	14585

Library-Prep
Sequencing

Library-Prep Information

	BGKcLV51-105	BGKcLV51-106
Sample #	19	20
Sample Name	SEPT7-BGKcLV51-105	SEPT7-BGKcLV51-106
Sample_Name_Alias
Index Well Position	C03	D03
Index_table	IDT_UniqueDualIndex_96_V2	IDT_UniqueDualIndex_96_V2
LibPrep_date	2026-05-18	2026-05-18
Lib_ID	Lib-260518	Lib-260518
Tecan_Location
Tecan
Tecan_date
Size_bp	291	288
Peak_Molarity	47.10	54.60
libSampleQC_DNA_Well	DNA_Library_set82/set1/C10.png	DNA_Library_set82/set1/D10.png
RIN	9.8	9.8
libSample_RNA_Well	RNA_Library_set82/set1/C3.png	RNA_Library_set82/set1/D3.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2026-05-21	2026-05-21
Sample_ID	BGKcLV51-105	BGKcLV51-106
RBP	SEPT7	SEPT7
Batch_ID	BGKcLV51	BGKcLV51
WB_result	53.100	56.600
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	4858	4859

Sequencing Information

	BGKcLV51-105	BGKcLV51-106
Sample_ID	BGKcLV51-105	BGKcLV51-106
Sample Name	SEPT7-BGKcLV51-105	SEPT7-BGKcLV51-106
Pool ID	Pool-260528	Pool-260528
LocalServer_folder	set82	set82
total_reads	38,077,347	45,406,311
total_aligned_reads	32,312,708	42,450,470
unique_aligned_reads	30,168,596	39,637,523
percent_uniqueAligned	0.79230	0.87295
correlation_replicates	0.999560	0.999560
spikein_reads	82,008	101,328
percent_spikeins	0.00215	0.00223
original_ReadLength	151	151
QC_Status	ReadyToSubmit	ReadyToSubmit
ID	3559	3560

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
SEPT7-BGKcLV51-105_R1.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set82/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3559	NovaSeq6000	100	paired-ended	1				NT-BGKcLV51-1_R1.filtered.trimmed.paired.fastq.gz,NT-BGKcLV51-2_R1.filtered.trimmed.paired.fastq.gz	ENCORE2	33700
SEPT7-BGKcLV51-105_R2.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set82/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3559	NovaSeq6000	100	paired-ended	2				NT-BGKcLV51-1_R2.filtered.trimmed.paired.fastq.gz,NT-BGKcLV51-2_R2.filtered.trimmed.paired.fastq.gz	ENCORE2	33758
SEPT7-BGKcLV51-105_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3559					GRCh38	V40	SEPT7-BGKcLV51-105_R1.filtered.trimmed.paired.fastq.gz,SEPT7-BGKcLV51-105_R2.filtered.trimmed.paired.fastq.gz		ENCORE2	33818
SEPT7-BGKcLV51-105_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3559					GRCh38	V40	SEPT7-BGKcLV51-105_Aligned.sortedByCoord.out.bam		ENCORE2	33876
SEPT7-BGKcLV51-105_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3559					GRCh38	V40	SEPT7-BGKcLV51-105_Aligned.sortedByCoord.out.bam		ENCORE2	33934
SEPT7-BGKcLV51-105_Signal.Unique.strand-.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3559					GRCh38	V40	SEPT7-BGKcLV51-105_Aligned.sortedByCoord.out.bam		ENCORE2	33992
SEPT7-BGKcLV51-105_Signal.Unique.strand+.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3559					GRCh38	V40	SEPT7-BGKcLV51-105_Aligned.sortedByCoord.out.bam		ENCORE2	34050
SEPT7-BGKcLV51-105_quant.sf	ENCODE_DATA/set82/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3559					GRCh38	V40	SEPT7-BGKcLV51-105_R1.filtered.trimmed.paired.fastq.gz,SEPT7-BGKcLV51-105_R2.filtered.trimmed.paired.fastq.gz		ENCORE2	34108
SEPT7-BGKcLV51-106_R1.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set82/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3560	NovaSeq6000	100	paired-ended	1				NT-BGKcLV51-1_R1.filtered.trimmed.paired.fastq.gz,NT-BGKcLV51-2_R1.filtered.trimmed.paired.fastq.gz	ENCORE2	33701
SEPT7-BGKcLV51-106_R2.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set82/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3560	NovaSeq6000	100	paired-ended	2				NT-BGKcLV51-1_R2.filtered.trimmed.paired.fastq.gz,NT-BGKcLV51-2_R2.filtered.trimmed.paired.fastq.gz	ENCORE2	33759
SEPT7-BGKcLV51-106_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3560					GRCh38	V40	SEPT7-BGKcLV51-106_R1.filtered.trimmed.paired.fastq.gz,SEPT7-BGKcLV51-106_R2.filtered.trimmed.paired.fastq.gz		ENCORE2	33819
SEPT7-BGKcLV51-106_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3560					GRCh38	V40	SEPT7-BGKcLV51-106_Aligned.sortedByCoord.out.bam		ENCORE2	33877
SEPT7-BGKcLV51-106_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3560					GRCh38	V40	SEPT7-BGKcLV51-106_Aligned.sortedByCoord.out.bam		ENCORE2	33935
SEPT7-BGKcLV51-106_Signal.Unique.strand-.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3560					GRCh38	V40	SEPT7-BGKcLV51-106_Aligned.sortedByCoord.out.bam		ENCORE2	33993
SEPT7-BGKcLV51-106_Signal.Unique.strand+.bw	ENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3560					GRCh38	V40	SEPT7-BGKcLV51-106_Aligned.sortedByCoord.out.bam		ENCORE2	34051
SEPT7-BGKcLV51-106_quant.sf	ENCODE_DATA/set82/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3560					GRCh38	V40	SEPT7-BGKcLV51-106_R1.filtered.trimmed.paired.fastq.gz,SEPT7-BGKcLV51-106_R2.filtered.trimmed.paired.fastq.gz		ENCORE2	34109