non-target      CRISPR in K562      Batch: BGKcLV51

General Information

List KD experiments controlled by BGKcLV51-1 and BGKcLV51-2


NT-BGKcLV51-1,NT-BGKcLV51-2:No KD experiments controlled by NT-BGKcLV51-1 and NT-BGKcLV51-2 are available on the database




Experiment Information (Status: Sequenced)
BGKcLV51-1BGKcLV51-2
idx00
TRCN#_or_BGC#BGC#0000000BGC#0000000
shRNA_or_gRNA_sequenceCAGTCGGGCGTCATCATGATCAGTCGGGCGTCATCATGAT
PAM
Namenon-targetnon-target
Sample_IDBGKcLV51-1BGKcLV51-2
transduction_Date9/30/20259/30/2025
daysD6D6
RBP_namenon-targetnon-target
qPCR_result
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc02418
WB_result
Ave_WB
WB_DONE_date
MW
IP
antibody_Cat#
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID62366237




Western Blot
Western Blot info was not avaliable




Experiment Status
BGKcLV51-1BGKcLV51-2
Sample_IDBGKcLV51-1BGKcLV51-2
Sample Name
Sample NameNT-BGKcLV51-1NT-BGKcLV51-2
RBPnon-targetnon-target
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2026-09-162026-09-16
ProjectENCORE2ENCORE2
Note
ID1456614567




Library-Prep Information
BGKcLV51-1BGKcLV51-2
Sample #12
Sample NameNT-BGKcLV51-1NT-BGKcLV51-2
Sample_Name_Alias
Index Well PositionA01B01
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2026-05-182026-05-18
Lib_IDLib-260518Lib-260518
Tecan_Location
Tecan
Tecan_date
Size_bp288297
Peak_Molarity47.0058.00
libSampleQC_DNA_WellDNA_Library_set82/set1/A8.pngDNA_Library_set82/set1/B8.png
RIN9.99.9
libSample_RNA_WellRNA_Library_set82/set1/A1.pngRNA_Library_set82/set1/B1.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2026-05-212026-05-21
Sample_IDBGKcLV51-1BGKcLV51-2
RBPNTNT
Batch_IDBGKcLV51BGKcLV51
WB_result0.0000.000
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID48404841




Sequencing Information
BGKcLV51-1BGKcLV51-2
Sample_IDBGKcLV51-1BGKcLV51-2
Sample NameNT-BGKcLV51-1NT-BGKcLV51-2
Pool IDPool-260528Pool-260528
LocalServer_folderset82set82
total_reads28,365,74332,273,579
total_aligned_reads24,053,13927,872,207
unique_aligned_reads22,437,11226,043,828
percent_uniqueAligned0.790990.80697
correlation_replicates0.9987610.998761
spikein_reads112,13457,292
percent_spikeins0.003950.00178
original_ReadLength151151
QC_StatusReadyToSubmitReadyToSubmit
ID35413542




Data Submission Information
DCC submission information was not avaliable




File Information
file_namefile_pathfile_formatoutput_typeassay_typelabSample IDplatformread_lengthrun_typepaired_endassemblygenome_annotationderived_fromcontrolled_byProjectNoteFILE_ID
NT-BGKcLV51-1_R1.filtered.trimmed.paired.fastq.gzENCODE_DATA/set82/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3541NovaSeq6000100paired-ended1ENCORE233680
NT-BGKcLV51-1_R2.filtered.trimmed.paired.fastq.gzENCODE_DATA/set82/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3541NovaSeq6000100paired-ended2ENCORE233738
NT-BGKcLV51-1_Aligned.sortedByCoord.out.bamENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbamalignmentsknockdown followed by RNA-seqGraveley Lab3541GRCh38V40NT-BGKcLV51-1_R1.filtered.trimmed.paired.fastq.gz,NT-BGKcLV51-1_R2.filtered.trimmed.paired.fastq.gzENCORE233796
NT-BGKcLV51-1_Signal.UniqueMultiple.strand-.bwENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbigWig-allminus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3541GRCh38V40NT-BGKcLV51-1_Aligned.sortedByCoord.out.bamENCORE233854
NT-BGKcLV51-1_Signal.UniqueMultiple.strand+.bwENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbigWig+allplus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3541GRCh38V40NT-BGKcLV51-1_Aligned.sortedByCoord.out.bamENCORE233912
NT-BGKcLV51-1_Signal.Unique.strand-.bwENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbigWig-uniqueminus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3541GRCh38V40NT-BGKcLV51-1_Aligned.sortedByCoord.out.bamENCORE233970
NT-BGKcLV51-1_Signal.Unique.strand+.bwENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbigWig+uniqueplus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3541GRCh38V40NT-BGKcLV51-1_Aligned.sortedByCoord.out.bamENCORE234028
NT-BGKcLV51-1_quant.sfENCODE_DATA/set82/salmon_ENCORE2_file_linkstranscript_quantificationtranscript quantificationsknockdown followed by RNA-seqGraveley Lab3541GRCh38V40NT-BGKcLV51-1_R1.filtered.trimmed.paired.fastq.gz,NT-BGKcLV51-1_R2.filtered.trimmed.paired.fastq.gzENCORE234086
NT-BGKcLV51-2_R1.filtered.trimmed.paired.fastq.gzENCODE_DATA/set82/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3542NovaSeq6000100paired-ended1ENCORE233681
NT-BGKcLV51-2_R2.filtered.trimmed.paired.fastq.gzENCODE_DATA/set82/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3542NovaSeq6000100paired-ended2ENCORE233739
NT-BGKcLV51-2_Aligned.sortedByCoord.out.bamENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbamalignmentsknockdown followed by RNA-seqGraveley Lab3542GRCh38V40NT-BGKcLV51-2_R1.filtered.trimmed.paired.fastq.gz,NT-BGKcLV51-2_R2.filtered.trimmed.paired.fastq.gzENCORE233797
NT-BGKcLV51-2_Signal.UniqueMultiple.strand-.bwENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbigWig-allminus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3542GRCh38V40NT-BGKcLV51-2_Aligned.sortedByCoord.out.bamENCORE233855
NT-BGKcLV51-2_Signal.UniqueMultiple.strand+.bwENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbigWig+allplus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3542GRCh38V40NT-BGKcLV51-2_Aligned.sortedByCoord.out.bamENCORE233913
NT-BGKcLV51-2_Signal.Unique.strand-.bwENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbigWig-uniqueminus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3542GRCh38V40NT-BGKcLV51-2_Aligned.sortedByCoord.out.bamENCORE233971
NT-BGKcLV51-2_Signal.Unique.strand+.bwENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbigWig+uniqueplus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3542GRCh38V40NT-BGKcLV51-2_Aligned.sortedByCoord.out.bamENCORE234029
NT-BGKcLV51-2_quant.sfENCODE_DATA/set82/salmon_ENCORE2_file_linkstranscript_quantificationtranscript quantificationsknockdown followed by RNA-seqGraveley Lab3542GRCh38V40NT-BGKcLV51-2_R1.filtered.trimmed.paired.fastq.gz,NT-BGKcLV51-2_R2.filtered.trimmed.paired.fastq.gzENCORE234087