SRP9      CRISPR in K562      Batch: BGKcLV48

Experiment Information (Status: LibPreping)
BGKcLV48-73BGKcLV48-74
idx00
TRCN#_or_BGC#BGC#0001052BGC#0001052
shRNA_or_gRNA_sequenceTTACCTTCATAGGGTCAGCGTTACCTTCATAGGGTCAGCG
PAMAGGAGG
NameSRP9_89SRP9_89
Sample_IDBGKcLV48-73BGKcLV48-74
transduction_Date4/15/254/15/25
daysD6D6
RBP_nameSRP9SRP9
qPCR_result0.00.0
Ave_qPCR0.0
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc22562338
WB_result65.160.9
Ave_WB
WB_DONE_date5/8/25
MW10kd
IP
antibody_Cat#A305-455Alot# 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID62046205




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SRP9Product_ID: A305-455A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SRP9-human
SRP9-HEPG2-CRISPR-A305-455A.png<br>Caption: Western blot following CRISPR against SRP9 in HepG2 whole cell lysate using SRP9 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SRP9. SRP9 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
SRP9-K562-CRISPR-A305-455A.png<br>Caption: Western blot following CRISPR against SRP9 in K562 whole cell lysate using SRP9 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SRP9. SRP9 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV48-73BGKcLV48-74
Sample_IDBGKcLV48-73BGKcLV48-74
Sample Name
Sample Name
RBPSRP9SRP9
Cell_LineK562K562
Exp UID
StatusLibPrepingLibPreping
Status_date2025-08-062025-08-06
ProjectENCORE2ENCORE2
Note
ID1425614257




Library-Prep Information
BGKcLV48-73BGKcLV48-74
Sample #1920
Sample NameSRP9-BGKcLV48-73SRP9-BGKcLV48-74
Sample_Name_Alias
Index Well PositionC03D03
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-182025-08-18
Lib_IDLib-250818Lib-250818
Tecan_Location
Tecan
Tecan_date
Size_bp264265
Peak_Molarity112.0089.50
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-08-192025-08-19
Sample_IDBGKcLV48-73BGKcLV48-74
RBPSRP9SRP9
Batch_IDBGKcLV48BGKcLV48
WB_result65.10060.900
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusWellPositionAssignedWellPositionAssigned
ProjectENCORE2ENCORE2
ID47644765




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database