SRP9      CRISPR in K562      Batch: BGKcLV48

Experiment Information (Status: Sequenced)
BGKcLV48-73BGKcLV48-74
idx00
TRCN#_or_BGC#BGC#0001052BGC#0001052
shRNA_or_gRNA_sequenceTTACCTTCATAGGGTCAGCGTTACCTTCATAGGGTCAGCG
PAMAGGAGG
NameSRP9_89SRP9_89
Sample_IDBGKcLV48-73BGKcLV48-74
transduction_Date4/15/254/15/25
daysD6D6
RBP_nameSRP9SRP9
qPCR_result0.00.0
Ave_qPCR0.0
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc22562338
WB_result65.160.9
Ave_WB
WB_DONE_date5/8/25
MW10kd
IP
antibody_Cat#A305-455Alot# 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID62066207




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SRP9Product_ID: A305-455A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SRP9-human		 SRP9-HEPG2-CRISPR-A305-455A.png<br>Caption: Western blot following CRISPR against SRP9 in HepG2 whole cell lysate using SRP9 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SRP9. SRP9 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 SRP9-K562-CRISPR-A305-455A.png<br>Caption: Western blot following CRISPR against SRP9 in K562 whole cell lysate using SRP9 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SRP9. SRP9 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV48-73BGKcLV48-74
Sample_IDBGKcLV48-73BGKcLV48-74
Sample Name
Sample NameSRP9-BGKcLV48-73SRP9-BGKcLV48-74
RBPSRP9SRP9
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2025-10-022025-10-02
ProjectENCORE2ENCORE2
Note
ID1425614257




Library-Prep Information
BGKcLV48-73BGKcLV48-74
Sample #1920
Sample NameSRP9-BGKcLV48-73SRP9-BGKcLV48-74
Sample_Name_Alias
Index Well PositionC03D03
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-182025-08-18
Lib_IDLib-250818Lib-250818
Tecan_Location
Tecan
Tecan_date
Size_bp264265
Peak_Molarity112.0089.50
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-08-192025-08-19
Sample_IDBGKcLV48-73BGKcLV48-74
RBPSRP9SRP9
Batch_IDBGKcLV48BGKcLV48
WB_result65.10060.900
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47644765




Sequencing Information
BGKcLV48-73BGKcLV48-74
Sample_IDBGKcLV48-73BGKcLV48-74
Sample NameSRP9-BGKcLV48-73SRP9-BGKcLV48-74
Pool IDPool-250822Pool-250822
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID34673468




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database