RPL8      CRISPR in K562      Batch: BGKcLV48

Experiment Information (Status: Sequencing)
BGKcLV48-63BGKcLV48-64
idx00
TRCN#_or_BGC#BGC#0001373BGC#0001373
shRNA_or_gRNA_sequenceGTGGATTTCGCTGAGCGGCAGTGGATTTCGCTGAGCGGCA
PAMCGGCGG
NameRPL8_93RPL8_93
Sample_IDBGKcLV48-63BGKcLV48-64
transduction_Date4/15/254/15/25
daysD6D6
RBP_nameRPL8RPL8
qPCR_result69.670.3
Ave_qPCR70.0
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc19251912
WB_result78.160.8
Ave_WB
WB_DONE_date5/14/25
MW28KD
IP
antibody_Cat#A305-059ALOT #1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID61946195




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RPL8Product_ID: A305-059A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RPL8-human		 RPL8-K562-CRISPR-A305-059A.png<br>Caption: Western blot following CRISPR against RPL8 in K562 whole cell lysate using RPL8 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPL8. RPL8 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV48-63BGKcLV48-64
Sample_IDBGKcLV48-63BGKcLV48-64
Sample Name
Sample NameRPL8-BGKcLV48-63RPL8-BGKcLV48-64
RBPRPL8RPL8
Cell_LineK562K562
Exp UID
StatusSequencingSequencing
Status_date2025-09-102025-09-10
ProjectENCORE2ENCORE2
Note
ID1425214253




Library-Prep Information
BGKcLV48-63BGKcLV48-64
Sample #1516
Sample NameRPL8-BGKcLV48-63RPL8-BGKcLV48-64
Sample_Name_Alias
Index Well PositionG02H02
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-182025-08-18
Lib_IDLib-250818Lib-250818
Tecan_Location
Tecan
Tecan_date
Size_bp264262
Peak_Molarity63.4058.10
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-08-192025-08-19
Sample_IDBGKcLV48-63BGKcLV48-64
RBPRPL8RPL8
Batch_IDBGKcLV48BGKcLV48
WB_result78.10060.800
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47604761




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database