RPL8      CRISPR in K562      Batch: BGKcLV48

Experiment Information (Status: Sequenced)
BGKcLV48-63BGKcLV48-64
idx00
TRCN#_or_BGC#BGC#0001373BGC#0001373
shRNA_or_gRNA_sequenceGTGGATTTCGCTGAGCGGCAGTGGATTTCGCTGAGCGGCA
PAMCGGCGG
NameRPL8_93RPL8_93
Sample_IDBGKcLV48-63BGKcLV48-64
transduction_Date4/15/254/15/25
daysD6D6
RBP_nameRPL8RPL8
qPCR_result69.670.3
Ave_qPCR70.0
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc19251912
WB_result78.160.8
Ave_WB
WB_DONE_date5/14/25
MW28KD
IP
antibody_Cat#A305-059ALOT #1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID61966197




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RPL8Product_ID: A305-059A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RPL8-human		 RPL8-K562-CRISPR-A305-059A.png<br>Caption: Western blot following CRISPR against RPL8 in K562 whole cell lysate using RPL8 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RPL8. RPL8 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV48-63BGKcLV48-64
Sample_IDBGKcLV48-63BGKcLV48-64
Sample Name
Sample NameRPL8-BGKcLV48-63RPL8-BGKcLV48-64
RBPRPL8RPL8
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2025-10-022025-10-02
ProjectENCORE2ENCORE2
Note
ID1425214253




Library-Prep Information
BGKcLV48-63BGKcLV48-64
Sample #1516
Sample NameRPL8-BGKcLV48-63RPL8-BGKcLV48-64
Sample_Name_Alias
Index Well PositionG02H02
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-182025-08-18
Lib_IDLib-250818Lib-250818
Tecan_Location
Tecan
Tecan_date
Size_bp264262
Peak_Molarity63.4058.10
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-08-192025-08-19
Sample_IDBGKcLV48-63BGKcLV48-64
RBPRPL8RPL8
Batch_IDBGKcLV48BGKcLV48
WB_result78.10060.800
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47604761




Sequencing Information
BGKcLV48-63BGKcLV48-64
Sample_IDBGKcLV48-63BGKcLV48-64
Sample NameRPL8-BGKcLV48-63RPL8-BGKcLV48-64
Pool IDPool-250822Pool-250822
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID34633464




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database