RNH1      CRISPR in K562      Batch: BGKcLV48

Experiment Information (Status: Sequencing)
BGKcLV48-59BGKcLV48-60
idx00
TRCN#_or_BGC#BGC#0001371BGC#0001371
shRNA_or_gRNA_sequenceCCTTGCACCGTGCTTCCGTGCCTTGCACCGTGCTTCCGTG
PAMAGGAGG
NameRNH1_90RNH1_90
Sample_IDBGKcLV48-59BGKcLV48-60
transduction_Date4/15/254/15/25
daysD6D6
RBP_nameRNH1RNH1
qPCR_result62.362.8
Ave_qPCR62.6
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc20082021
WB_result58.056.7
Ave_WB
WB_DONE_date5/14/25
MW50KD
IP
antibody_Cat#A305-357ALOT #1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID61906191




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RNH1Product_ID: A305-357A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RNH1-human		 RNH1-HEPG2-A305-357A.png<br>Caption: Western blot following CRISPR against RNH1 in HepG2 whole cell lysate using RNH1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RNH1. RNH1 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.
 Bethyl_A305-357A_1_RNH1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RNH1 specific antibody, A305-357A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RNH1-specific antibody, A305-357A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 RNH1-K562-CRISPR-A305-357A.png<br>Caption: Western blot following CRISPR against RNH1 in K562 whole cell lysate using RNH1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RNH1. RNH1 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.




Experiment Status
BGKcLV48-59BGKcLV48-60
Sample_IDBGKcLV48-59BGKcLV48-60
Sample Name
Sample NameRNH1-BGKcLV48-59RNH1-BGKcLV48-60
RBPRNH1RNH1
Cell_LineK562K562
Exp UID
StatusSequencingSequencing
Status_date2025-09-102025-09-10
ProjectENCORE2ENCORE2
Note
ID1425014251




Library-Prep Information
BGKcLV48-59BGKcLV48-60
Sample #1314
Sample NameRNH1-BGKcLV48-59RNH1-BGKcLV48-60
Sample_Name_Alias
Index Well PositionE02F02
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-182025-08-18
Lib_IDLib-250818Lib-250818
Tecan_Location
Tecan
Tecan_date
Size_bp262261
Peak_Molarity108.0074.10
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-08-192025-08-19
Sample_IDBGKcLV48-59BGKcLV48-60
RBPRNH1RNH1
Batch_IDBGKcLV48BGKcLV48
WB_result58.00056.700
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47584759




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database