RBM27      CRISPR in K562      Batch: BGKcLV48

Experiment Information (Status: LibPreping)
BGKcLV48-47BGKcLV48-48
idx00
TRCN#_or_BGC#BGC#0001360BGC#0001360
shRNA_or_gRNA_sequenceTGTTGTAGCACTGGTCAAGATGTTGTAGCACTGGTCAAGA
PAMAGGAGG
NameRBM27_79RBM27_79
Sample_IDBGKcLV48-47BGKcLV48-48
transduction_Date4/15/254/15/25
daysD6D6
RBP_nameRBM27RBM27
qPCR_result51.948.4
Ave_qPCR50.2
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc18662008
WB_result64.463.4
Ave_WB
WB_DONE_date5/7/25
MW119KD
IP
antibody_Cat#A301-234ALOT #1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID61766177




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RBM27Product_ID: A301-234A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RBM27-human		 Bethyl_A301-234A_1_RBM27.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RBM27 specific antibody, A301-234A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RBM27-specific antibody, A301-234A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 RBM27-K562-CRISPR-A301-234A.png<br>Caption: Western blot following CRISPR against RBM27 in K562 whole cell lysate using RBM27 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RBM27. RBM27 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV48-47BGKcLV48-48
Sample_IDBGKcLV48-47BGKcLV48-48
Sample Name
Sample Name
RBPRBM27RBM27
Cell_LineK562K562
Exp UID
StatusLibPrepingLibPreping
Status_date2025-08-062025-08-06
ProjectENCORE2ENCORE2
Note
ID1424614247




Library-Prep Information
BGKcLV48-47BGKcLV48-48
Sample #910
Sample NameRBM27-BGKcLV48-47RBM27-BGKcLV48-48
Sample_Name_Alias
Index Well PositionA02B02
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-182025-08-18
Lib_IDLib-250818Lib-250818
Tecan_Location
Tecan
Tecan_date
Size_bp274284
Peak_Molarity132.00131.00
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-08-192025-08-19
Sample_IDBGKcLV48-47BGKcLV48-48
RBPRBM27RBM27
Batch_IDBGKcLV48BGKcLV48
WB_result64.40063.400
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusWellPositionAssignedWellPositionAssigned
ProjectENCORE2ENCORE2
ID47544755




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database