RBM27      CRISPR in K562      Batch: BGKcLV48

Experiment Information (Status: Sequenced)
BGKcLV48-47BGKcLV48-48
idx00
TRCN#_or_BGC#BGC#0001360BGC#0001360
shRNA_or_gRNA_sequenceTGTTGTAGCACTGGTCAAGATGTTGTAGCACTGGTCAAGA
PAMAGGAGG
NameRBM27_79RBM27_79
Sample_IDBGKcLV48-47BGKcLV48-48
transduction_Date4/15/254/15/25
daysD6D6
RBP_nameRBM27RBM27
qPCR_result51.948.4
Ave_qPCR50.2
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc18662008
WB_result64.463.4
Ave_WB
WB_DONE_date5/7/25
MW119KD
IP
antibody_Cat#A301-234ALOT #1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID61786179




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RBM27Product_ID: A301-234A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RBM27-human		 Bethyl_A301-234A_1_RBM27.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RBM27 specific antibody, A301-234A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RBM27-specific antibody, A301-234A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 RBM27-K562-CRISPR-A301-234A.png<br>Caption: Western blot following CRISPR against RBM27 in K562 whole cell lysate using RBM27 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RBM27. RBM27 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV48-47BGKcLV48-48
Sample_IDBGKcLV48-47BGKcLV48-48
Sample Name
Sample NameRBM27-BGKcLV48-47RBM27-BGKcLV48-48
RBPRBM27RBM27
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2025-10-022025-10-02
ProjectENCORE2ENCORE2
Note
ID1424614247




Library-Prep Information
BGKcLV48-47BGKcLV48-48
Sample #910
Sample NameRBM27-BGKcLV48-47RBM27-BGKcLV48-48
Sample_Name_Alias
Index Well PositionA02B02
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-182025-08-18
Lib_IDLib-250818Lib-250818
Tecan_Location
Tecan
Tecan_date
Size_bp274284
Peak_Molarity132.00131.00
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-08-192025-08-19
Sample_IDBGKcLV48-47BGKcLV48-48
RBPRBM27RBM27
Batch_IDBGKcLV48BGKcLV48
WB_result64.40063.400
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47544755




Sequencing Information
BGKcLV48-47BGKcLV48-48
Sample_IDBGKcLV48-47BGKcLV48-48
Sample NameRBM27-BGKcLV48-47RBM27-BGKcLV48-48
Pool IDPool-250822Pool-250822
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID34573458




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database