RBM27 RNA-Seq in K562

Experiment Information (Status: Sequenced)

	BGKcLV48-47	BGKcLV48-48
idx	0	0
TRCN#_or_BGC#	BGC#0001360	BGC#0001360
shRNA_or_gRNA_sequence	TGTTGTAGCACTGGTCAAGA	TGTTGTAGCACTGGTCAAGA
PAM	AGG	AGG
Name	RBM27_79	RBM27_79
Sample_ID	BGKcLV48-47	BGKcLV48-48
transduction_Date	4/15/25	4/15/25
days	D6	D6
RBP_name	RBM27	RBM27
qPCR_result	51.9	48.4
Ave_qPCR	50.2
RT-qPCR_primer-F	agcctgagccaaaaccacta
RT-qPCR_primer-R	tctgccatctcgttcttcct
protein_conc	1866	2008
WB_result	64.4	63.4
Ave_WB
WB_DONE_date	5/7/25
MW	119KD
IP
antibody_Cat#	A301-234A	LOT #1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note
ID	6193	6194

Western Blot

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
RBM27	Product_ID: A301-234A Lot_ID: 1 Source: Bethyl Labs Target Name: RBM27-human

Experiment Status

	BGKcLV48-47	BGKcLV48-48
Sample_ID	BGKcLV48-47	BGKcLV48-48
Sample Name
Sample Name	RBM27-BGKcLV48-47	RBM27-BGKcLV48-48
RBP	RBM27	RBM27
Cell_Line	K562	K562
Exp UID
Status	Sequenced	Sequenced
Status_date	2025-10-02	2025-10-02
Project	ENCORE2	ENCORE2
Note
ID	14246	14247

Library-Prep
Sequencing

Library-Prep Information

	BGKcLV48-47	BGKcLV48-48
Sample #	9	10
Sample Name	RBM27-BGKcLV48-47	RBM27-BGKcLV48-48
Sample_Name_Alias
Index Well Position	A02	B02
Index_table	IDT_UniqueDualIndex_96_V2	IDT_UniqueDualIndex_96_V2
LibPrep_date	2025-08-18	2025-08-18
Lib_ID	Lib-250818	Lib-250818
Tecan_Location
Tecan
Tecan_date
Size_bp	274	284
Peak_Molarity	132.00	131.00
libSampleQC_DNA_Well	DNA_Library_set79/set1/A2.png	DNA_Library_set79/set1/B2.png
RIN	10.0	10.0
libSample_RNA_Well	RNA_Library_set79/set1/A11.png	RNA_Library_set79/set1/B11.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2025-08-19	2025-08-19
Sample_ID	BGKcLV48-47	BGKcLV48-48
RBP	RBM27	RBM27
Batch_ID	BGKcLV48	BGKcLV48
WB_result	64.400	63.400
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	4754	4755

Sequencing Information

	BGKcLV48-47	BGKcLV48-48
Sample_ID	BGKcLV48-47	BGKcLV48-48
Sample Name	RBM27-BGKcLV48-47	RBM27-BGKcLV48-48
Pool ID	Pool-250822	Pool-250822
LocalServer_folder	set79	set79
total_reads	16,470,523	17,719,586
total_aligned_reads	14,505,624	15,576,473
unique_aligned_reads	13,447,944	14,444,749
percent_uniqueAligned	0.81649	0.81519
correlation_replicates	0.999124	0.999124
spikein_reads	76,916	74,340
percent_spikeins	0.00467	0.00420
original_ReadLength	151	151
QC_Status	ReadyToSubmit	ReadyToSubmit
ID	3457	3458

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
RBM27-BGKcLV48-47_S173_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set79/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3457	NovaSeq6000	100	paired-ended	1				NT-BGKcLV48-1_S165_L002_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGKcLV48-2_S166_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	32828
RBM27-BGKcLV48-47_S173_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set79/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3457	NovaSeq6000	100	paired-ended	2				NT-BGKcLV48-1_S165_L002_R2_001.filtered.trimmed.paired.fastq.gz,NT-BGKcLV48-2_S166_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	32850
RBM27-BGKcLV48-47_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3457					GRCh38	V40	RBM27-BGKcLV48-47_S173_L002_R1_001.filtered.trimmed.paired.fastq.gz,RBM27-BGKcLV48-47_S173_L002_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	32872
RBM27-BGKcLV48-47_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3457					GRCh38	V40	RBM27-BGKcLV48-47_Aligned.sortedByCoord.out.bam		ENCORE2	32894
RBM27-BGKcLV48-47_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3457					GRCh38	V40	RBM27-BGKcLV48-47_Aligned.sortedByCoord.out.bam		ENCORE2	32916
RBM27-BGKcLV48-47_Signal.Unique.strand-.bw	ENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3457					GRCh38	V40	RBM27-BGKcLV48-47_Aligned.sortedByCoord.out.bam		ENCORE2	32938
RBM27-BGKcLV48-47_Signal.Unique.strand+.bw	ENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3457					GRCh38	V40	RBM27-BGKcLV48-47_Aligned.sortedByCoord.out.bam		ENCORE2	32960
RBM27-BGKcLV48-48_S174_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set79/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3458	NovaSeq6000	100	paired-ended	1				NT-BGKcLV48-1_S165_L002_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGKcLV48-2_S166_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	32829
RBM27-BGKcLV48-48_S174_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set79/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3458	NovaSeq6000	100	paired-ended	2				NT-BGKcLV48-1_S165_L002_R2_001.filtered.trimmed.paired.fastq.gz,NT-BGKcLV48-2_S166_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	32851
RBM27-BGKcLV48-48_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3458					GRCh38	V40	RBM27-BGKcLV48-48_S174_L002_R1_001.filtered.trimmed.paired.fastq.gz,RBM27-BGKcLV48-48_S174_L002_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	32873
RBM27-BGKcLV48-48_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3458					GRCh38	V40	RBM27-BGKcLV48-48_Aligned.sortedByCoord.out.bam		ENCORE2	32895
RBM27-BGKcLV48-48_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3458					GRCh38	V40	RBM27-BGKcLV48-48_Aligned.sortedByCoord.out.bam		ENCORE2	32917
RBM27-BGKcLV48-48_Signal.Unique.strand-.bw	ENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3458					GRCh38	V40	RBM27-BGKcLV48-48_Aligned.sortedByCoord.out.bam		ENCORE2	32939
RBM27-BGKcLV48-48_Signal.Unique.strand+.bw	ENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3458					GRCh38	V40	RBM27-BGKcLV48-48_Aligned.sortedByCoord.out.bam		ENCORE2	32961