NUFIP2      CRISPR in K562      Batch: BGKcLV48

Experiment Information (Status: Sequenced)
BGKcLV48-41BGKcLV48-42
idx00
TRCN#_or_BGC#BGC#0001036BGC#0001036
shRNA_or_gRNA_sequenceGTCCCAGCCGCTTTCAATGGGTCCCAGCCGCTTTCAATGG
PAMAGGAGG
NameNUFIP2_88NUFIP2_88
Sample_IDBGKcLV48-41BGKcLV48-42
transduction_Date4/15/254/15/25
daysD6D6
RBP_nameNUFIP2NUFIP2
qPCR_result33.617.7
Ave_qPCR25.7
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc15461624
WB_result66.064.8
Ave_WB
WB_DONE_date5/1/25
MW76kd
IP
antibody_Cat#A301-600Alot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID61726173




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
NUFIP2Product_ID: A301-600A
Lot_ID: 1
Source: Bethyl Labs
Target Name: NUFIP2-human		 NUFIP2-K562-CRISPR-A301-600A.png<br>Caption: Western blot following CRISPR against NUFIP2 in K562 whole cell lysate using NUFIP2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against NUFIP2. NUFIP2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV48-41BGKcLV48-42
Sample_IDBGKcLV48-41BGKcLV48-42
Sample Name
Sample NameNUFIP2-BGKcLV48-41NUFIP2-BGKcLV48-42
RBPNUFIP2NUFIP2
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2025-10-022025-10-02
ProjectENCORE2ENCORE2
Note
ID1424414245




Library-Prep Information
BGKcLV48-41BGKcLV48-42
Sample #78
Sample NameNUFIP2-BGKcLV48-41NUFIP2-BGKcLV48-42
Sample_Name_Alias
Index Well PositionG01H01
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-182025-08-18
Lib_IDLib-250818Lib-250818
Tecan_Location
Tecan
Tecan_date
Size_bp254254
Peak_Molarity50.5041.40
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-08-192025-08-19
Sample_IDBGKcLV48-41BGKcLV48-42
RBPNUFIP2NUFIP2
Batch_IDBGKcLV48BGKcLV48
WB_result66.00064.800
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47524753




Sequencing Information
BGKcLV48-41BGKcLV48-42
Sample_IDBGKcLV48-41BGKcLV48-42
Sample NameNUFIP2-BGKcLV48-41NUFIP2-BGKcLV48-42
Pool IDPool-250822Pool-250822
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID34553456




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database