HNRNPD      CRISPR in K562      Batch: BGKcLV48

Experiment Information (Status: Sequenced)
BGKcLV48-31BGKcLV48-32
idx00
TRCN#_or_BGC#BGC#0000192BGC#0000192
shRNA_or_gRNA_sequenceGTCGGAGGAGCAGTTCGGCGGTCGGAGGAGCAGTTCGGCG
PAMGGGGGG
NameHNRNPD-93FHNRNPD-93F
Sample_IDBGKcLV48-31BGKcLV48-32
transduction_Date4/15/254/15/25
daysD6D6
RBP_nameHNRNPDHNRNPD
qPCR_result38.026.7
Ave_qPCR32.4
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc23992552
WB_result84.886.3
Ave_WB
WB_DONE_date5/1/25
MW38kdTUBULIN
IPfu's #18
antibody_Cat##12382lot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID61626163




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
HNRNPDProduct_ID: 12382S
Lot_ID: 1
Source: Cell Signaling
Target Name: HNRNPD-human		 HNRNPD-K562-CRISPR-#12382S.png<br>Caption: Western blot following CRISPR against HNRNPD in K562 whole cell lysate using HNRNPD specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against HNRNPD. HNRNPD protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV48-31BGKcLV48-32
Sample_IDBGKcLV48-31BGKcLV48-32
Sample Name
Sample NameHNRNPD-BGKcLV48-31HNRNPD-BGKcLV48-32
RBPHNRNPDHNRNPD
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2025-10-022025-10-02
ProjectENCORE2ENCORE2
Note
ID1424214243




Library-Prep Information
BGKcLV48-31BGKcLV48-32
Sample #56
Sample NameHNRNPD-BGKcLV48-31HNRNPD-BGKcLV48-32
Sample_Name_Alias
Index Well PositionE01F01
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-182025-08-18
Lib_IDLib-250818Lib-250818
Tecan_Location
Tecan
Tecan_date
Size_bp264248
Peak_Molarity85.9092.10
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-08-192025-08-19
Sample_IDBGKcLV48-31BGKcLV48-32
RBPHNRNPDHNRNPD
Batch_IDBGKcLV48BGKcLV48
WB_result84.80086.300
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47504751




Sequencing Information
BGKcLV48-31BGKcLV48-32
Sample_IDBGKcLV48-31BGKcLV48-32
Sample NameHNRNPD-BGKcLV48-31HNRNPD-BGKcLV48-32
Pool IDPool-250822Pool-250822
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID34533454




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database