HNRNPD      CRISPR in K562      Batch: BGKcLV48

Experiment Information (Status: Sequencing)
BGKcLV48-31BGKcLV48-32
idx00
TRCN#_or_BGC#BGC#0000192BGC#0000192
shRNA_or_gRNA_sequenceGTCGGAGGAGCAGTTCGGCGGTCGGAGGAGCAGTTCGGCG
PAMGGGGGG
NameHNRNPD-93FHNRNPD-93F
Sample_IDBGKcLV48-31BGKcLV48-32
transduction_Date4/15/254/15/25
daysD6D6
RBP_nameHNRNPDHNRNPD
qPCR_result38.026.7
Ave_qPCR32.4
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc23992552
WB_result84.886.3
Ave_WB
WB_DONE_date5/1/25
MW38kdTUBULIN
IPfu's #18
antibody_Cat##12382lot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID61606161




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
HNRNPDProduct_ID: 12382S
Lot_ID: 1
Source: Cell Signaling
Target Name: HNRNPD-human		 HNRNPD-K562-CRISPR-#12382S.png<br>Caption: Western blot following CRISPR against HNRNPD in K562 whole cell lysate using HNRNPD specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against HNRNPD. HNRNPD protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV48-31BGKcLV48-32
Sample_IDBGKcLV48-31BGKcLV48-32
Sample Name
Sample NameHNRNPD-BGKcLV48-31HNRNPD-BGKcLV48-32
RBPHNRNPDHNRNPD
Cell_LineK562K562
Exp UID
StatusSequencingSequencing
Status_date2025-09-102025-09-10
ProjectENCORE2ENCORE2
Note
ID1424214243




Library-Prep Information
BGKcLV48-31BGKcLV48-32
Sample #56
Sample NameHNRNPD-BGKcLV48-31HNRNPD-BGKcLV48-32
Sample_Name_Alias
Index Well PositionE01F01
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-182025-08-18
Lib_IDLib-250818Lib-250818
Tecan_Location
Tecan
Tecan_date
Size_bp264248
Peak_Molarity85.9092.10
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-08-192025-08-19
Sample_IDBGKcLV48-31BGKcLV48-32
RBPHNRNPDHNRNPD
Batch_IDBGKcLV48BGKcLV48
WB_result84.80086.300
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47504751




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database