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RBM5 RNA-Seq in K562



RBM5      CRISPR in K562      Control:

General Information
RBPRBM5
Cell_LineK562
MethodCRISPR
Exp_NameRBM5-BGKcLV44-K562
ENCODE_series_ID
Batch_IDBGKcLV44
Pool IDPool-241015
Local_Set_Nameset75
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1RBM5-BGKcLV44-99
Rep2RBM5-BGKcLV44-100
CN1NT-BGKcLV44-1
CN2NT-BGKcLV44-2
Rep1_qPCR0.0
Rep2_qPCR0.0
Rep1_WB63.7
Rep2_WB53.5
Antibody Cat#86425S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1614




Experiment Information (Status: Sequenced)
BGKcLV44-99BGKcLV44-100
idx00
TRCN#_or_BGC#BGC#0000042BGC#0000042
shRNA_or_gRNA_sequenceACTATGGTGAGCACGACTATACTATGGTGAGCACGACTAT
PAM
NameRBM5_4-FRBM5_4-F
Sample_IDBGKcLV44-99BGKcLV44-100
transduction_Date7/30/247/30/24
daysD6D6
RBP_nameRBM5RBM5
qPCR_result0.00.0
Ave_qPCR0.0
RT-qPCR_primer-FCCTTAGCTGAAAGGCAGCAG
RT-qPCR_primer-RCACCAGCTCCTCCTCATTGT
protein_conc46744710
WB_result63.753.5
Ave_WB58.6
WB_DONE_date8/22/24,8/28/24
MW92kDa
IP
antibody_Cat#86425Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID59805981




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RBM5Product_ID: 86425S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: RBM5-human		 CST_86425S_1_RBM5.png<br>Caption: IP-WB analysis of 86425S whole cell lysate using the RBM5 specific antibody, 86425S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RBM5-specific antibody, 86425S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 RBM5-K562-CRISPR-86425S.png<br>Caption: Western blot following CRISPR against RBM5 in K562 whole cell lysate using RBM5 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RBM5. RBM5 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV44-99BGKcLV44-100
Sample_IDBGKcLV44-99BGKcLV44-100
Sample Name
Sample NameRBM5-BGKcLV44-99RBM5-BGKcLV44-100
RBPRBM5RBM5
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-11-062024-11-06
ProjectENCORE2ENCORE2
Note
ID1362013621




Library-Prep Information
BGKcLV44-99BGKcLV44-100
Sample #4950
Sample NameRBM5-BGKcLV44-99RBM5-BGKcLV44-100
Sample_Name_Alias
Index Well PositionA12B12
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2024-10-102024-10-10
Lib_IDLib-241010Lib-241010
Tecan_Location
Tecan
Tecan_date
Size_bp286278
Peak_Molarity68.1095.40
libSampleQC_DNA_WellDNA_Library_set75/set1/A12.pngDNA_Library_set75/set1/B12.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set75/set1/A6.pngRNA_Library_set75/set1/B6.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-10-152024-10-15
Sample_IDBGKcLV44-99BGKcLV44-100
RBPRBM5RBM5
Batch_IDBGKcLV44BGKcLV44
WB_result63.70053.500
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID46084609




Sequencing Information
BGKcLV44-99BGKcLV44-100
Sample_IDBGKcLV44-99BGKcLV44-100
Sample NameRBM5-BGKcLV44-99RBM5-BGKcLV44-100
Pool IDPool-241015Pool-241015
LocalServer_folderset75set75
total_reads46,046,13452,381,057
total_aligned_reads43,375,27149,991,480
unique_aligned_reads39,841,31846,059,836
percent_uniqueAligned0.865250.87932
correlation_replicates0.9722340.972234
spikein_reads71,72262,334
percent_spikeins0.001560.00119
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID33193320




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database