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YARS RNA-Seq in K562



YARS      CRISPR in K562      Control:

General Information
RBPYARS
Cell_LineK562
MethodCRISPR
Exp_NameYARS-BGKcLV44-K562
ENCODE_series_ID
Batch_IDBGKcLV44
Pool IDPool-241015
Local_Set_Nameset75
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1YARS-BGKcLV44-85
Rep2YARS-BGKcLV44-86
CN1NT-BGKcLV44-1
CN2NT-BGKcLV44-2
Rep1_qPCR62.5
Rep2_qPCR62.4
Rep1_WB63.4
Rep2_WB60.5
Antibody Cat#A305-064A
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1613




Experiment Information (Status: Sequenced)
BGKcLV44-85BGKcLV44-86
idx00
TRCN#_or_BGC#BGC#0001216BGC#0001216
shRNA_or_gRNA_sequenceAGAAACTGCACCTTATCACCAGAAACTGCACCTTATCACC
PAMCGGCGG
NameYARS_82YARS_82
Sample_IDBGKcLV44-85BGKcLV44-86
transduction_Date7/30/247/30/24
daysD6D6
RBP_nameYARSYARS
qPCR_result62.562.4
Ave_qPCR62.5
RT-qPCR_primer-Fctccctgcacttggctattc
RT-qPCR_primer-Rccttccgatcaaggagatca
protein_conc33214670
WB_result63.460.5
Ave_WB61.9
WB_DONE_date8/19/24,8/28/24
MW59kd
IP
antibody_Cat#A305-064Alot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID59665967




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
YARSProduct_ID: A305-064A
Lot_ID: 1
Source: Bethyl Labs
Target Name: YARS-human		 YARS-HEPG2-CRISPR-A305-064A.png<br>Caption: Western blot following CRISPR against YARS in HepG2 whole cell lysate using YARS specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against YARS. YARS protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 YARS-K562-CRISPR-A305-064A.png<br>Caption: Western blot following CRISPR against YARS in K562 whole cell lysate using YARS specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against YARS. YARS protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV44-85BGKcLV44-86
Sample_IDBGKcLV44-85BGKcLV44-86
Sample Name
Sample NameYARS-BGKcLV44-85YARS-BGKcLV44-86
RBPYARSYARS
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-11-062024-11-06
ProjectENCORE2ENCORE2
Note
ID1361813619




Library-Prep Information
BGKcLV44-85BGKcLV44-86
Sample #4748
Sample NameYARS-BGKcLV44-85YARS-BGKcLV44-86
Sample_Name_Alias
Index Well PositionG11H11
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2024-10-102024-10-10
Lib_IDLib-241010Lib-241010
Tecan_Location
Tecan
Tecan_date
Size_bp286288
Peak_Molarity77.3079.90
libSampleQC_DNA_WellDNA_Library_set75/set1/G11.pngDNA_Library_set75/set1/H11.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set75/set1/G5.pngRNA_Library_set75/set1/H5.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-10-152024-10-15
Sample_IDBGKcLV44-85BGKcLV44-86
RBPYARSYARS
Batch_IDBGKcLV44BGKcLV44
WB_result63.40060.500
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID46064607




Sequencing Information
BGKcLV44-85BGKcLV44-86
Sample_IDBGKcLV44-85BGKcLV44-86
Sample NameYARS-BGKcLV44-85YARS-BGKcLV44-86
Pool IDPool-241015Pool-241015
LocalServer_folderset75set75
total_reads49,706,85158,686,921
total_aligned_reads47,879,38556,162,914
unique_aligned_reads44,452,33752,082,784
percent_uniqueAligned0.894290.88747
correlation_replicates0.9993400.999340
spikein_reads53,796109,074
percent_spikeins0.001080.00186
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID33173318




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database