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WARS RNA-Seq in K562



WARS      CRISPR in K562      Control:

General Information
RBPWARS
Cell_LineK562
MethodCRISPR
Exp_NameWARS-BGKcLV44-K562
ENCODE_series_ID
Batch_IDBGKcLV44
Pool IDPool-241015
Local_Set_Nameset75
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1WARS-BGKcLV44-83
Rep2WARS-BGKcLV44-84
CN1NT-BGKcLV44-1
CN2NT-BGKcLV44-2
Rep1_qPCR70.3
Rep2_qPCR76.5
Rep1_WB75.4
Rep2_WB79.5
Antibody Cat#A304-274A
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1612




Experiment Information (Status: Sequenced)
BGKcLV44-83BGKcLV44-84
idx00
TRCN#_or_BGC#BGC#0001075BGC#0001075
shRNA_or_gRNA_sequenceGAGCTACAAAGCTGCCGCGGGAGCTACAAAGCTGCCGCGG
PAMGGGGGG
NameWARS_94BWARS_94B
Sample_IDBGKcLV44-83BGKcLV44-84
transduction_Date7/30/247/30/24
daysD6D6
RBP_nameWARSWARS
qPCR_result70.376.5
Ave_qPCR73.4
RT-qPCR_primer-Fcgcagaggcatcttcttctc
RT-qPCR_primer-Rcggcccgtgtacagataaaa
protein_conc42964956
WB_result75.479.5
Ave_WB77.5
WB_DONE_date8/19/24
MW53KD
IP
antibody_Cat#A304-274Alot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID59645965




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
WARSProduct_ID: A304-274A
Lot_ID: 1
Source: Bethyl Labs
Target Name: WARS-human		 WARS-HEPG2-CRISPR-A304-274A.png<br>Caption: Western blot following CRISPR against WARS in HepG2 whole cell lysate using WARS specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against WARS. WARS protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 WARS-K562-CRISPR-A304-274A.png<br>Caption: Western blot following CRISPR against WARS in K562 whole cell lysate using WARS specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against WARS. WARS protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV44-83BGKcLV44-84
Sample_IDBGKcLV44-83BGKcLV44-84
Sample Name
Sample NameWARS-BGKcLV44-83WARS-BGKcLV44-84
RBPWARSWARS
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-11-062024-11-06
ProjectENCORE2ENCORE2
Note
ID1361613617




Library-Prep Information
BGKcLV44-83BGKcLV44-84
Sample #4546
Sample NameWARS-BGKcLV44-83WARS-BGKcLV44-84
Sample_Name_Alias
Index Well PositionE11F11
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2024-10-102024-10-10
Lib_IDLib-241010Lib-241010
Tecan_Location
Tecan
Tecan_date
Size_bp289290
Peak_Molarity70.0065.00
libSampleQC_DNA_WellDNA_Library_set75/set1/E11.pngDNA_Library_set75/set1/F11.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set75/set1/E5.pngRNA_Library_set75/set1/F5.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-10-152024-10-15
Sample_IDBGKcLV44-83BGKcLV44-84
RBPWARSWARS
Batch_IDBGKcLV44BGKcLV44
WB_result75.40079.500
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID46044605




Sequencing Information
BGKcLV44-83BGKcLV44-84
Sample_IDBGKcLV44-83BGKcLV44-84
Sample NameWARS-BGKcLV44-83WARS-BGKcLV44-84
Pool IDPool-241015Pool-241015
LocalServer_folderset75set75
total_reads52,411,70450,791,871
total_aligned_reads50,106,57148,922,765
unique_aligned_reads46,547,32945,038,981
percent_uniqueAligned0.888110.88674
correlation_replicates0.9971660.997166
spikein_reads65,73064,418
percent_spikeins0.001250.00127
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID33153316




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database