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SSR1 RNA-Seq in K562



SSR1      CRISPR in K562      Control:

General Information
RBPSSR1
Cell_LineK562
MethodCRISPR
Exp_NameSSR1-BGKcLV44-K562
ENCODE_series_ID
Batch_IDBGKcLV44
Pool IDPool-241015
Local_Set_Nameset75
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1SSR1-BGKcLV44-73
Rep2SSR1-BGKcLV44-74
CN1NT-BGKcLV44-1
CN2NT-BGKcLV44-2
Rep1_qPCR45.5
Rep2_qPCR45.0
Rep1_WB49.8
Rep2_WB58.3
Antibody Cat#A304-978A
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1611




Experiment Information (Status: Sequenced)
BGKcLV44-73BGKcLV44-74
idx00
TRCN#_or_BGC#BGC#0001219BGC#0001219
shRNA_or_gRNA_sequenceAGTGGCACAAGATCTTACAGAGTGGCACAAGATCTTACAG
PAMAGGAGG
NameSSR1_81SSR1_81
Sample_IDBGKcLV44-73BGKcLV44-74
transduction_Date7/30/247/30/24
daysD6D6
RBP_nameSSR1SSR1
qPCR_result45.545.0
Ave_qPCR45.3
RT-qPCR_primer-Fagcttcaccgagtgcagata
RT-qPCR_primer-Rgaccaaaccaaatggtcgtc
protein_conc44304914
WB_result49.858.3
Ave_WB54.1
WB_DONE_date8/14/24
MW
IP
antibody_Cat#A304-978Alot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID59545955




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SSR1Product_ID: A304-978A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SSR1-human		 SSR1-K562-CRISPR-A304-978A.png<br>Caption: Western blot following CRISPR against SSR1 in K562 whole cell lysate using SSR1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SSR1. SSR1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV44-73BGKcLV44-74
Sample_IDBGKcLV44-73BGKcLV44-74
Sample Name
Sample NameSSR1-BGKcLV44-73SSR1-BGKcLV44-74
RBPSSR1SSR1
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-11-062024-11-06
ProjectENCORE2ENCORE2
Note
ID1361413615




Library-Prep Information
BGKcLV44-73BGKcLV44-74
Sample #4344
Sample NameSSR1-BGKcLV44-73SSR1-BGKcLV44-74
Sample_Name_Alias
Index Well PositionC11D11
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2024-10-102024-10-10
Lib_IDLib-241010Lib-241010
Tecan_Location
Tecan
Tecan_date
Size_bp288295
Peak_Molarity75.4079.50
libSampleQC_DNA_WellDNA_Library_set75/set1/C11.pngDNA_Library_set75/set1/D11.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set75/set1/C5.pngRNA_Library_set75/set1/D5.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-10-152024-10-15
Sample_IDBGKcLV44-73BGKcLV44-74
RBPSSR1SSR1
Batch_IDBGKcLV44BGKcLV44
WB_result49.80058.300
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID46024603




Sequencing Information
BGKcLV44-73BGKcLV44-74
Sample_IDBGKcLV44-73BGKcLV44-74
Sample NameSSR1-BGKcLV44-73SSR1-BGKcLV44-74
Pool IDPool-241015Pool-241015
LocalServer_folderset75set75
total_reads51,479,70154,758,083
total_aligned_reads48,973,27652,106,225
unique_aligned_reads45,423,53148,327,017
percent_uniqueAligned0.882360.88255
correlation_replicates0.9976130.997613
spikein_reads74,03466,502
percent_spikeins0.001440.00121
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID33133314




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database