Notice: Undefined offset: 1 in /website/encore_alpha/gene/index.php on line 599
CPSF1 RNA-Seq in K562



CPSF1      CRISPR in K562      Control:

General Information
RBPCPSF1
Cell_LineK562
MethodCRISPR
Exp_NameCPSF1-BGKcLV44-K562
ENCODE_series_ID
Batch_IDBGKcLV44
Pool IDPool-241015
Local_Set_Nameset75
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1CPSF1-BGKcLV44-7
Rep2CPSF1-BGKcLV44-8
CN1NT-BGKcLV44-1
CN2NT-BGKcLV44-2
Rep1_qPCR0.0
Rep2_qPCR18.0
Rep1_WB70.3
Rep2_WB66.8
Antibody Cat#A301-580A
Antibody Lot#lot # 2
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1596




Experiment Information (Status: Sequenced)
BGKcLV44-7BGKcLV44-8
idx00
TRCN#_or_BGC#BGC#0001083BGC#0001083
shRNA_or_gRNA_sequenceAAGCTCGAGCTTCTCCCGGTAAGCTCGAGCTTCTCCCGGT
PAMgggggg
NameCPSF1-2CPSF1-2
Sample_IDBGKcLV44-7BGKcLV44-8
transduction_Date7/30/247/30/24
daysD6D6
RBP_nameCPSF1CPSF1
qPCR_result0.018.0
Ave_qPCR9.0
RT-qPCR_primer-Factggcgaggagaaggagtt
RT-qPCR_primer-Rgatgagctggatggagaagg
protein_conc51675434
WB_result70.366.8
Ave_WB68.6
WB_DONE_date8/7/24,8/21/24
MW
IP
antibody_Cat#A301-580Alot # 2
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID58825883




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
CPSF1Product_ID: A301-580A
Lot_ID: 2
Source: Bethyl Labs
Target Name: CPSF1-human		 Bethyl_A301-580A_2_CPSF1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CPSF1 specific antibody, A301-580A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CPSF1-specific antibody, A301-580A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 CPSF1-K562-CRISPR-A301-580A.png<br>Caption: Western blot following CRISPR against CPSF1 in K562 whole cell lysate using CPSF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CPSF1. CPSF1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV44-7BGKcLV44-8
Sample_IDBGKcLV44-7BGKcLV44-8
Sample Name
Sample NameCPSF1-BGKcLV44-7CPSF1-BGKcLV44-8
RBPCPSF1CPSF1
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-11-062024-11-06
ProjectENCORE2ENCORE2
Note
ID1358413585




Library-Prep Information
BGKcLV44-7BGKcLV44-8
Sample #1314
Sample NameCPSF1-BGKcLV44-7CPSF1-BGKcLV44-8
Sample_Name_Alias
Index Well PositionE07F07
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2024-10-102024-10-10
Lib_IDLib-241010Lib-241010
Tecan_Location
Tecan
Tecan_date
Size_bp280283
Peak_Molarity70.4087.20
libSampleQC_DNA_WellDNA_Library_set75/set1/E7.pngDNA_Library_set75/set1/F7.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set75/set1/E1.pngRNA_Library_set75/set1/F1.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-10-152024-10-15
Sample_IDBGKcLV44-7BGKcLV44-8
RBPCPSF1CPSF1
Batch_IDBGKcLV44BGKcLV44
WB_result70.30066.800
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID45724573




Sequencing Information
BGKcLV44-7BGKcLV44-8
Sample_IDBGKcLV44-7BGKcLV44-8
Sample NameCPSF1-BGKcLV44-7CPSF1-BGKcLV44-8
Pool IDPool-241015Pool-241015
LocalServer_folderset75set75
total_reads54,975,94155,180,544
total_aligned_reads49,615,15751,844,681
unique_aligned_reads45,627,36547,915,978
percent_uniqueAligned0.829950.86835
correlation_replicates0.9925150.992515
spikein_reads118,99288,662
percent_spikeins0.002160.00161
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID32833284




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database