Notice: Undefined offset: 1 in /website/encore_alpha/gene/index.php on line 599
SRSF10 RNA-Seq in K562



SRSF10      CRISPR in K562      Control:

General Information
RBPSRSF10
Cell_LineK562
MethodCRISPR
Exp_NameSRSF10-BGKcLV44-K562
ENCODE_series_ID
Batch_IDBGKcLV44
Pool IDPool-241015
Local_Set_Nameset75
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1SRSF10-BGKcLV44-69
Rep2SRSF10-BGKcLV44-70
CN1NT-BGKcLV44-1
CN2NT-BGKcLV44-2
Rep1_qPCR10.0
Rep2_qPCR22.7
Rep1_WB79.0
Rep2_WB76.8
Antibody Cat#RN064PW/4
Antibody Lot#
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1610




Experiment Information (Status: Sequenced)
BGKcLV44-69BGKcLV44-70
idx00
TRCN#_or_BGC#BGC#0000098BGC#0000098
shRNA_or_gRNA_sequenceGGAACGTGGCCGACGACACCGGAACGTGGCCGACGACACC
PAMAGGAGG
NameSRSF10_91B-1SRSF10_91B-1
Sample_IDBGKcLV44-69BGKcLV44-70
transduction_Date7/30/247/30/24
daysD6D6
RBP_nameSRSF10SRSF10
qPCR_result10.022.7
Ave_qPCR16.4
RT-qPCR_primer-FCCCCCAACACGTCTCTGTT
RT-qPCR_primer-RAAATTCACGCCGCAAGTCT
protein_conc48784258
WB_result79.076.8
Ave_WB
WB_DONE_date8/14/24,8/28/24
MW
IP
antibody_Cat#RN064PW/4
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID59505951




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SRSF10Product_ID: RN064PW
Lot_ID: 001
Source: MBLI
Target Name: SRSF10-human		 SRSF10-K562-CRISPR-RN064PW.png<br>Caption: Western blot following CRISPR against SRSF10 in K562 whole cell lysate using SRSF10 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SRSF10. SRSF10 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV44-69BGKcLV44-70
Sample_IDBGKcLV44-69BGKcLV44-70
Sample Name
Sample NameSRSF10-BGKcLV44-69SRSF10-BGKcLV44-70
RBPSRSF10SRSF10
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-11-062024-11-06
ProjectENCORE2ENCORE2
Note
ID1361213613




Library-Prep Information
BGKcLV44-69BGKcLV44-70
Sample #4142
Sample NameSRSF10-BGKcLV44-69SRSF10-BGKcLV44-70
Sample_Name_Alias
Index Well PositionA11B11
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2024-10-102024-10-10
Lib_IDLib-241010Lib-241010
Tecan_Location
Tecan
Tecan_date
Size_bp288287
Peak_Molarity65.9074.90
libSampleQC_DNA_WellDNA_Library_set75/set1/A11.pngDNA_Library_set75/set1/B11.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set75/set1/A5.pngRNA_Library_set75/set1/B5.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-10-152024-10-15
Sample_IDBGKcLV44-69BGKcLV44-70
RBPSRSF10SRSF10
Batch_IDBGKcLV44BGKcLV44
WB_result79.00076.800
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID46004601




Sequencing Information
BGKcLV44-69BGKcLV44-70
Sample_IDBGKcLV44-69BGKcLV44-70
Sample NameSRSF10-BGKcLV44-69SRSF10-BGKcLV44-70
Pool IDPool-241015Pool-241015
LocalServer_folderset75set75
total_reads63,820,03446,536,027
total_aligned_reads61,005,09443,397,168
unique_aligned_reads56,512,59540,176,585
percent_uniqueAligned0.885500.86334
correlation_replicates0.9955780.995578
spikein_reads162,994106,952
percent_spikeins0.002550.00230
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID33113312




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database