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RANGAP1 RNA-Seq in K562



RANGAP1      CRISPR in K562      Control:

General Information
RBPRANGAP1
Cell_LineK562
MethodCRISPR
Exp_NameRANGAP1-BGKcLV44-K562
ENCODE_series_ID
Batch_IDBGKcLV44
Pool IDPool-241015
Local_Set_Nameset75
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1RANGAP1-BGKcLV44-51
Rep2RANGAP1-BGKcLV44-52
CN1NT-BGKcLV44-1
CN2NT-BGKcLV44-2
Rep1_qPCR57.0
Rep2_qPCR52.4
Rep1_WB62.7
Rep2_WB62.4
Antibody Cat#36067S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1606




Experiment Information (Status: Sequenced)
BGKcLV44-51BGKcLV44-51BGKcLV44-52BGKcLV44-52
idx0000
TRCN#_or_BGC#BGC#0001048BGC#0001048BGC#0001048BGC#0001048
shRNA_or_gRNA_sequenceGCCAAGACTCAGGTGGCCGGGCCAAGACTCAGGTGGCCGGGCCAAGACTCAGGTGGCCGGGCCAAGACTCAGGTGGCCGG
PAMgggggggggggg
NameRANGAP1_82RANGAP1_82RANGAP1_82RANGAP1_82
Sample_IDBGKcLV44-51BGKcLV44-51BGKcLV44-52BGKcLV44-52
transduction_Date7/30/247/30/247/30/247/30/24
daysD6D6D6D6
RBP_nameRANGAP1RANGAP1RANGAP1RANGAP1
qPCR_result57.052.4
Ave_qPCR54.7
RT-qPCR_primer-FGGTGGTCTCTGCCTTCCTAAAG
RT-qPCR_primer-RCGAGGAGTTGAAAGCCTTCTGC
protein_conc4257425753315331
WB_result62.784.562.485.5
Ave_WB62.585.0
WB_DONE_date8/12/248/12/24
MW82KD68KD
IP
antibody_Cat#36067S36067Slot # 1lot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM0000
Rep2_TPM0000
ActionReadyReady
Library_start_date
repeat_library
Note
ID5928593059295931




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RANGAP1Product_ID: 36067S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: RANGAP1-human		 CST_36067S_1_RANGAP1.png<br>Caption: IP-WB analysis of 36067S whole cell lysate using the RANGAP1 specific antibody, 36067S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RANGAP1-specific antibody, 36067S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 RANGAP1-K562-CRISPR-36067S.png<br>Caption: Western blot following CRISPR against RANGAP1 in K562 whole cell lysate using RANGAP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RANGAP1. RANGAP1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV44-51BGKcLV44-51BGKcLV44-52BGKcLV44-52
Sample_IDBGKcLV44-51BGKcLV44-51BGKcLV44-52BGKcLV44-52
Sample Name
Sample NameRANGAP1-BGKcLV44-51RANGAP1-BGKcLV44-52
RBPRANGAP1RANGAP1RANGAP1RANGAP1
Cell_LineK562K562K562K562
Exp UID
StatusSequencedNotSatisfiedSequencedNotSatisfied
Status_date2024-11-062024-10-082024-11-062024-10-08
ProjectENCORE2ENCORE2ENCORE2ENCORE2
Note
ID13604136481360513649




Library-Prep Information
BGKcLV44-51BGKcLV44-52
Sample #3334
Sample NameRANGAP1-BGKcLV44-51RANGAP1-BGKcLV44-52
Sample_Name_Alias
Index Well PositionA10B10
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2024-10-102024-10-10
Lib_IDLib-241010Lib-241010
Tecan_Location
Tecan
Tecan_date
Size_bp296295
Peak_Molarity82.8085.10
libSampleQC_DNA_WellDNA_Library_set75/set1/A10.pngDNA_Library_set75/set1/B10.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set75/set1/A4.pngRNA_Library_set75/set1/B4.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-10-152024-10-15
Sample_IDBGKcLV44-51BGKcLV44-52
RBPRANGAP1RANGAP1
Batch_IDBGKcLV44BGKcLV44
WB_result62.70062.400
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID45924593




Sequencing Information
BGKcLV44-51BGKcLV44-52
Sample_IDBGKcLV44-51BGKcLV44-52
Sample NameRANGAP1-BGKcLV44-51RANGAP1-BGKcLV44-52
Pool IDPool-241015Pool-241015
LocalServer_folderset75set75
total_reads32,612,01751,007,165
total_aligned_reads30,921,54848,516,774
unique_aligned_reads28,471,84844,876,771
percent_uniqueAligned0.873050.87981
correlation_replicates0.9902680.990268
spikein_reads84,55099,378
percent_spikeins0.002590.00195
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID33033304




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database