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EIF5B RNA-Seq in K562



EIF5B      CRISPR in K562      Control:

General Information
RBPEIF5B
Cell_LineK562
MethodCRISPR
Exp_NameEIF5B-BGKcLV44-K562
ENCODE_series_ID
Batch_IDBGKcLV44
Pool IDPool-241015
Local_Set_Nameset75
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1EIF5B-BGKcLV44-25
Rep2EIF5B-BGKcLV44-26
CN1NT-BGKcLV44-1
CN2NT-BGKcLV44-2
Rep1_qPCR5.1
Rep2_qPCR16.7
Rep1_WB69.2
Rep2_WB67.4
Antibody Cat#A301-745A
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1600




Experiment Information (Status: Sequenced)
BGKcLV44-25BGKcLV44-25BGKcLV44-26BGKcLV44-26
idx0000
TRCN#_or_BGC#BGC#0000985BGC#0000985BGC#0000985BGC#0000985
shRNA_or_gRNA_sequenceAGCGCCATTGACAAGCAATGAGCGCCATTGACAAGCAATGAGCGCCATTGACAAGCAATGAGCGCCATTGACAAGCAATG
PAMGGGGGGGGGGGG
NameEIF5B_81EIF5B_81EIF5B_81EIF5B_81
Sample_IDBGKcLV44-25BGKcLV44-25BGKcLV44-26BGKcLV44-26
transduction_Date7/30/247/30/247/30/247/30/24
daysD6D6D6D6
RBP_nameEIF5BEIF5BEIF5BEIF5B
qPCR_result5.15.116.716.7
Ave_qPCR10.910.9
RT-qPCR_primer-Fagagagaagccagagccagaagagagaagccagagccaga
RT-qPCR_primer-Rtttgatggcacttcaacacctttgatggcacttcaacacc
protein_conc4496449641634163
WB_result69.267.067.453.7
Ave_WB68.367.0
WB_DONE_date8/13/241/7/24
MW139kd139kd
IP
antibody_Cat#A301-745AA301-744Alot # 1lot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM0000
Rep2_TPM0000
ActionReadyReady
Library_start_date
repeat_library
Note
ID5900590259015903




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EIF5BProduct_ID: A301-745A
Lot_ID: 1
Source: Bethyl Labs
Target Name: EIF5B-human		 EIF5B-K562-CRISPR-A301-745A.png<br>Caption: Western blot following CRISPR against EIF5B in K562 whole cell lysate using EIF5B specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF5B. EIF5B protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV44-25BGKcLV44-26
Sample_IDBGKcLV44-25BGKcLV44-26
Sample Name
Sample NameEIF5B-BGKcLV44-25EIF5B-BGKcLV44-26
RBPEIF5BEIF5B
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-11-062024-11-06
ProjectENCORE2ENCORE2
Note
ID1359213593




Library-Prep Information
BGKcLV44-25BGKcLV44-26
Sample #2122
Sample NameEIF5B-BGKcLV44-25EIF5B-BGKcLV44-26
Sample_Name_Alias
Index Well PositionE08F08
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2024-10-102024-10-10
Lib_IDLib-241010Lib-241010
Tecan_Location
Tecan
Tecan_date
Size_bp293283
Peak_Molarity94.7072.70
libSampleQC_DNA_WellDNA_Library_set75/set1/E8.pngDNA_Library_set75/set1/F8.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set75/set1/E2.pngRNA_Library_set75/set1/F2.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-10-152024-10-15
Sample_IDBGKcLV44-25BGKcLV44-26
RBPEIF5BEIF5B
Batch_IDBGKcLV44BGKcLV44
WB_result69.20067.400
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID45804581




Sequencing Information
BGKcLV44-25BGKcLV44-26
Sample_IDBGKcLV44-25BGKcLV44-26
Sample NameEIF5B-BGKcLV44-25EIF5B-BGKcLV44-26
Pool IDPool-241015Pool-241015
LocalServer_folderset75set75
total_reads38,375,48038,868,281
total_aligned_reads36,677,68936,850,175
unique_aligned_reads34,006,25334,228,093
percent_uniqueAligned0.886150.88062
correlation_replicates0.9929930.992993
spikein_reads76,67655,058
percent_spikeins0.002000.00142
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID32913292




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database