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EIF2S1 RNA-Seq in K562



EIF2S1      CRISPR in K562      Control:

General Information
RBPEIF2S1
Cell_LineK562
MethodCRISPR
Exp_NameEIF2S1-BGKcLV44-K562
ENCODE_series_ID
Batch_IDBGKcLV44
Pool IDPool-241015
Local_Set_Nameset75
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1EIF2S1-BGKcLV44-23
Rep2EIF2S1-BGKcLV44-24
CN1NT-BGKcLV44-1
CN2NT-BGKcLV44-2
Rep1_qPCR83.0
Rep2_qPCR79.2
Rep1_WB60.1
Rep2_WB60.9
Antibody Cat#5324S
Antibody Lot#lot # 9
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1599




Experiment Information (Status: Sequenced)
BGKcLV44-23BGKcLV44-24
idx00
TRCN#_or_BGC#BGC#0001138BGC#0001138
shRNA_or_gRNA_sequenceTGAGGTGTTAGAATACACCATGAGGTGTTAGAATACACCA
PAMAGGAGG
NameEIF2S1_77EIF2S1_77
Sample_IDBGKcLV44-23BGKcLV44-24
transduction_Date7/30/247/30/24
daysD6D6
RBP_nameEIF2S1EIF2S1
qPCR_result83.079.2
Ave_qPCR81.1
RT-qPCR_primer-FTTGAATGAAGATGAACGGGAA
RT-qPCR_primer-RGCATCAATGCCTTCATAACCA
protein_conc48304557
WB_result60.160.9
Ave_WB60.5
WB_DONE_date8/8/24
MW38kd
IP
antibody_Cat#5324Slot # 9
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID58985899




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EIF2S1Product_ID: 5324S
Lot_ID: 9
Source: Cell Signaling Technology
Target Name: EIF2S1-human		 CST_5324S_9_EIF2S1.png<br>Caption: IP-WB analysis of 5324S whole cell lysate using the EIF2S1 specific antibody, 5324S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the EIF2S1-specific antibody, 5324S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 EIF2S1-K562-CRISPR-5324S.png<br>Caption: Western blot following CRISPR against EIF2S1 in K562 whole cell lysate using EIF2S1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF2S1. EIF2S1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV44-23BGKcLV44-24
Sample_IDBGKcLV44-23BGKcLV44-24
Sample Name
Sample NameEIF2S1-BGKcLV44-23EIF2S1-BGKcLV44-24
RBPEIF2S1EIF2S1
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-11-062024-11-06
ProjectENCORE2ENCORE2
Note
ID1359013591




Library-Prep Information
BGKcLV44-23BGKcLV44-24
Sample #1920
Sample NameEIF2S1-BGKcLV44-23EIF2S1-BGKcLV44-24
Sample_Name_Alias
Index Well PositionC08D08
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2024-10-102024-10-10
Lib_IDLib-241010Lib-241010
Tecan_Location
Tecan
Tecan_date
Size_bp292292
Peak_Molarity102.0070.30
libSampleQC_DNA_WellDNA_Library_set75/set1/C8.pngDNA_Library_set75/set1/D8.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set75/set1/C2.pngRNA_Library_set75/set1/D2.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-10-152024-10-15
Sample_IDBGKcLV44-23BGKcLV44-24
RBPEIF2S1EIF2S1
Batch_IDBGKcLV44BGKcLV44
WB_result60.10060.900
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID45784579




Sequencing Information
BGKcLV44-23BGKcLV44-24
Sample_IDBGKcLV44-23BGKcLV44-24
Sample NameEIF2S1-BGKcLV44-23EIF2S1-BGKcLV44-24
Pool IDPool-241015Pool-241015
LocalServer_folderset75set75
total_reads46,895,75545,633,725
total_aligned_reads43,986,26942,930,891
unique_aligned_reads40,769,33439,829,984
percent_uniqueAligned0.869360.87282
correlation_replicates0.9992100.999210
spikein_reads93,27283,126
percent_spikeins0.001990.00182
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID32893290




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database