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ESS2 RNA-Seq in K562



ESS2      CRISPR in K562      Control:

General Information
RBPESS2
Cell_LineK562
MethodCRISPR
Exp_NameESS2-BGKcLV44-K562
ENCODE_series_ID
Batch_IDBGKcLV44
Pool IDPool-241015
Local_Set_Nameset75
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1ESS2-BGKcLV44-19
Rep2ESS2-BGKcLV44-20
CN1NT-BGKcLV44-1
CN2NT-BGKcLV44-2
Rep1_qPCR0.0
Rep2_qPCR0.0
Rep1_WB63.8
Rep2_WB57.5
Antibody Cat#A304-566A
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1598




Experiment Information (Status: Sequenced)
BGKcLV44-19BGKcLV44-20
idx00
TRCN#_or_BGC#BGC#0000909BGC#0000909
shRNA_or_gRNA_sequenceCAGGCCCCCGAGGAAGCGCGCAGGCCCCCGAGGAAGCGCG
PAMaggagg
NameDGCR14_82DGCR14_82
Sample_IDBGKcLV44-19BGKcLV44-20
transduction_Date7/30/247/30/24
daysD6D6
RBP_nameESS2ESS2
qPCR_result0.00.0
Ave_qPCR0.0
RT-qPCR_primer-Ftccaaatgaatttgcaaaacttac
RT-qPCR_primer-Rtctcctcttcttcccctgct
protein_conc42124516
WB_result63.857.5
Ave_WB60.7
WB_DONE_date8/8/24
MW53kd
IP
antibody_Cat#A304-566Alot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID58945895




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
ESS2Product_ID: A304-566A
Lot_ID: 1
Source: Bethyl Labs
Target Name: ESS2-human		 DGCR14-K562-CRISPR-A304-566A.png<br>Caption: Western blot following CRISPR against ESS2 in K562 whole cell lysate using ESS2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ESS2. ESS2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV44-19BGKcLV44-20
Sample_IDBGKcLV44-19BGKcLV44-20
Sample Name
Sample NameESS2-BGKcLV44-19ESS2-BGKcLV44-20
RBPESS2ESS2
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-11-062024-11-06
ProjectENCORE2ENCORE2
Note
ID1358813589




Library-Prep Information
BGKcLV44-19BGKcLV44-20
Sample #1718
Sample NameESS2-BGKcLV44-19ESS2-BGKcLV44-20
Sample_Name_Alias
Index Well PositionA08B08
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2024-10-102024-10-10
Lib_IDLib-241010Lib-241010
Tecan_Location
Tecan
Tecan_date
Size_bp288296
Peak_Molarity72.7080.70
libSampleQC_DNA_WellDNA_Library_set75/set1/A8.pngDNA_Library_set75/set1/B8.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set75/set1/A2.pngRNA_Library_set75/set1/B2.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-10-152024-10-15
Sample_IDBGKcLV44-19BGKcLV44-20
RBPESS2ESS2
Batch_IDBGKcLV44BGKcLV44
WB_result63.80057.500
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID45764577




Sequencing Information
BGKcLV44-19BGKcLV44-20
Sample_IDBGKcLV44-19BGKcLV44-20
Sample NameESS2-BGKcLV44-19ESS2-BGKcLV44-20
Pool IDPool-241015Pool-241015
LocalServer_folderset75set75
total_reads68,710,74256,112,441
total_aligned_reads64,893,45353,015,652
unique_aligned_reads59,730,65849,084,278
percent_uniqueAligned0.869310.87475
correlation_replicates0.9936150.993615
spikein_reads173,094146,510
percent_spikeins0.002520.00261
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID32873288




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database