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UBA2 RNA-Seq in K562



UBA2      CRISPR in K562      Control:

General Information
RBPUBA2
Cell_LineK562
MethodCRISPR
Exp_NameUBA2-BGKcLV42-K562
ENCODE_series_ID
Batch_IDBGKcLV42
Pool IDPool-240715
Local_Set_Nameset73
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1UBA2-BGKcLV42-97
Rep2UBA2-BGKcLV42-98
CN1NT-BGKcLV42-1
CN2NT-BGKcLV42-2
Rep1_qPCR71.3
Rep2_qPCR70.4
Rep1_WB95.8
Rep2_WB95.1
Antibody Cat#8688S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1564




Experiment Information (Status: Sequenced)
BGKcLV42-97BGKcLV42-98
idx00
TRCN#_or_BGC#BGC#0001196BGC#0001196
shRNA_or_gRNA_sequenceGTGTTACGAATTGTACAGCCGTGTTACGAATTGTACAGCC
PAMAGGAGG
NameUBA2_84UBA2_84
Sample_IDBGKcLV42-97BGKcLV42-98
transduction_Date4/23/244/23/24
daysD6D6
RBP_nameUBA2UBA2
qPCR_result71.370.4
Ave_qPCR70.9
RT-qPCR_primer-Fggaatgggctaaatcaactgg
RT-qPCR_primer-Racggaactggaggtttcctt
protein_conc25832814
WB_result95.895.1
Ave_WB95.4
WB_DONE_date5/10/24
MW90kd
IP
antibody_Cat#8688Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID58635864




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
UBA2Product_ID: 8688S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: UBA2-human		 CST_8688S_1_UBA2.png<br>Caption: IP-WB analysis of 8688S whole cell lysate using the UBA2 specific antibody, 8688S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the UBA2-specific antibody, 8688S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 UBA2-K562-CRISPR-8688S.png<br>Caption: Western blot following CRISPR against UBA2 in K562 whole cell lysate using UBA2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against UBA2. UBA2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV42-97BGKcLV42-98
Sample_IDBGKcLV42-97BGKcLV42-98
Sample Name
Sample NameUBA2-BGKcLV42-97UBA2-BGKcLV42-98
RBPUBA2UBA2
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1312213123




Library-Prep Information
BGKcLV42-97BGKcLV42-98
Sample #7980
Sample NameUBA2-BGKcLV42-97UBA2-BGKcLV42-98
Sample_Name_Alias
Index Well PositionG10H10
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-06-212024-06-21
Lib_IDLib-240621Lib-240621
Tecan_Location
Tecan
Tecan_date
Size_bp328315
Peak_Molarity130.0081.60
libSampleQC_DNA_WellDNA_Library_set73/set1/G10.pngDNA_Library_set73/set1/H10.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set73/set1/G10.pngRNA_Library_set73/set1/H10.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-152024-07-15
Sample_IDBGKcLV42-97BGKcLV42-98
RBPUBA2UBA2
Batch_IDBGKcLV42BGKcLV42
WB_result95.80095.100
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID43484349




Sequencing Information
BGKcLV42-97BGKcLV42-98
Sample_IDBGKcLV42-97BGKcLV42-98
Sample NameUBA2-BGKcLV42-97UBA2-BGKcLV42-98
Pool IDPool-240715Pool-240715
LocalServer_folderset73set73
total_reads69,856,38344,060,860
total_aligned_reads64,354,09842,080,503
unique_aligned_reads59,666,47138,982,733
percent_uniqueAligned0.854130.88475
correlation_replicates0.9923950.992395
spikein_reads8,2004,994
percent_spikeins0.000120.00011
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID32153216




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database