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SSU72 RNA-Seq in K562



SSU72      CRISPR in K562      Control:

General Information
RBPSSU72
Cell_LineK562
MethodCRISPR
Exp_NameSSU72-BGKcLV42-K562
ENCODE_series_ID
Batch_IDBGKcLV42
Pool IDPool-240715
Local_Set_Nameset73
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1SSU72-BGKcLV42-89
Rep2SSU72-BGKcLV42-90
CN1NT-BGKcLV42-1
CN2NT-BGKcLV42-2
Rep1_qPCR81.0
Rep2_qPCR80.3
Rep1_WB78.0
Rep2_WB82.6
Antibody Cat#12816S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1561




Experiment Information (Status: Sequenced)
BGKcLV42-89BGKcLV42-90
idx00
TRCN#_or_BGC#BGC#0001189BGC#0001189
shRNA_or_gRNA_sequenceGCGTCCGATCCTTTGGAACAGCGTCCGATCCTTTGGAACA
PAMGGGGGG
NameSSU72_91SSU72_91
Sample_IDBGKcLV42-89BGKcLV42-90
transduction_Date4/23/244/23/24
daysD6D6
RBP_nameSSU72SSU72
qPCR_result81.080.3
Ave_qPCR80.7
RT-qPCR_primer-Fcaaccagaaccggagcat
RT-qPCR_primer-Ragcttcacgtgagtccctgt
protein_conc21561987
WB_result78.082.6
Ave_WB80.3
WB_DONE_date5/8/24
MW27kd
IP
antibody_Cat#12816Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID58555856




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SSU72Product_ID: 12816S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: SSU72-human		 SSU72-HEPG2-CRISPR-12816S.png<br>Caption: Western blot following CRISPR against SSU72 in HepG2 whole cell lysate using SSU72 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SSU72. SSU72 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_12816S_1_SSU72.png<br>Caption: IP-WB analysis of 12816S whole cell lysate using the SSU72 specific antibody, 12816S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the SSU72-specific antibody, 12816S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 SSU72-K562-CRISPR-12816S.png<br>Caption: Western blot following CRISPR against SSU72 in K562 whole cell lysate using SSU72 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SSU72. SSU72 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV42-89BGKcLV42-90
Sample_IDBGKcLV42-89BGKcLV42-90
Sample Name
Sample NameSSU72-BGKcLV42-89SSU72-BGKcLV42-90
RBPSSU72SSU72
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1311613117




Library-Prep Information
BGKcLV42-89BGKcLV42-90
Sample #7374
Sample NameSSU72-BGKcLV42-89SSU72-BGKcLV42-90
Sample_Name_Alias
Index Well PositionA10B10
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-06-212024-06-21
Lib_IDLib-240621Lib-240621
Tecan_Location
Tecan
Tecan_date
Size_bp333317
Peak_Molarity71.5087.60
libSampleQC_DNA_WellDNA_Library_set73/set1/A10.pngDNA_Library_set73/set1/B10.png
RIN9.910.0
libSample_RNA_WellRNA_Library_set73/set1/A10.pngRNA_Library_set73/set1/B10.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-152024-07-15
Sample_IDBGKcLV42-89BGKcLV42-90
RBPSSU72SSU72
Batch_IDBGKcLV42BGKcLV42
WB_result78.00082.600
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID43424343




Sequencing Information
BGKcLV42-89BGKcLV42-90
Sample_IDBGKcLV42-89BGKcLV42-90
Sample NameSSU72-BGKcLV42-89SSU72-BGKcLV42-90
Pool IDPool-240715Pool-240715
LocalServer_folderset73set73
total_reads50,601,51138,363,790
total_aligned_reads47,832,06433,621,773
unique_aligned_reads44,089,25831,011,311
percent_uniqueAligned0.871300.80835
correlation_replicates0.9942440.994244
spikein_reads6,9041,394
percent_spikeins0.000140.00004
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID32093210




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database