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SEC24D RNA-Seq in K562



SEC24D      CRISPR in K562      Control:

General Information
RBPSEC24D
Cell_LineK562
MethodCRISPR
Exp_NameSEC24D-BGKcLV42-K562
ENCODE_series_ID
Batch_IDBGKcLV42
Pool IDPool-240715
Local_Set_Nameset73
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1SEC24D-BGKcLV42-83
Rep2SEC24D-BGKcLV42-84
CN1NT-BGKcLV42-1
CN2NT-BGKcLV42-2
Rep1_qPCR76.4
Rep2_qPCR69.6
Rep1_WB73.9
Rep2_WB81.6
Antibody Cat#14687S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1559




Experiment Information (Status: Sequenced)
BGKcLV42-83BGKcLV42-84
idx00
TRCN#_or_BGC#BGC#0001183BGC#0001183
shRNA_or_gRNA_sequenceTTGGGGGCCACCGCCACTAGTTGGGGGCCACCGCCACTAG
PAMGGGGGG
NameSEC24D_92SEC24D_92
Sample_IDBGKcLV42-83BGKcLV42-84
transduction_Date4/23/244/23/24
daysD6D6
RBP_nameSEC24DSEC24D
qPCR_result76.469.6
Ave_qPCR73.0
RT-qPCR_primer-Fagaatggagctcatgccact
RT-qPCR_primer-Rgatgccacattgttgacagg
protein_conc21952798
WB_result73.981.6
Ave_WB77.8
WB_DONE_date5/10/24
MW115kd
IP
antibody_Cat#14687Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID58495850




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SEC24DProduct_ID: 14687S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: SEC24D-human		 SEC24D-HEPG2-CRISPR-14687S.png<br>Caption: Western blot following CRISPR against SEC24D in HepG2 whole cell lysate using SEC24D specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SEC24D. SEC24D protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_14687S_1_SEC24D.png<br>Caption: IP-WB analysis of 14687S whole cell lysate using the SEC24D specific antibody, 14687S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the SEC24D-specific antibody, 14687S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 SEC24D-K562-CRISPR-14687S.png<br>Caption: Western blot following CRISPR against SEC24D in K562 whole cell lysate using SEC24D specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SEC24D. SEC24D protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV42-83BGKcLV42-84
Sample_IDBGKcLV42-83BGKcLV42-84
Sample Name
Sample NameSEC24D-BGKcLV42-83SEC24D-BGKcLV42-84
RBPSEC24DSEC24D
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1311213113




Library-Prep Information
BGKcLV42-83BGKcLV42-84
Sample #6970
Sample NameSEC24D-BGKcLV42-83SEC24D-BGKcLV42-84
Sample_Name_Alias
Index Well PositionE09F09
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-06-212024-06-21
Lib_IDLib-240621Lib-240621
Tecan_Location
Tecan
Tecan_date
Size_bp301305
Peak_Molarity61.4092.90
libSampleQC_DNA_WellDNA_Library_set73/set1/E9.pngDNA_Library_set73/set1/F9.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set73/set1/E9.pngRNA_Library_set73/set1/F9.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-152024-07-15
Sample_IDBGKcLV42-83BGKcLV42-84
RBPSEC24DSEC24D
Batch_IDBGKcLV42BGKcLV42
WB_result73.90081.600
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID43384339




Sequencing Information
BGKcLV42-83BGKcLV42-84
Sample_IDBGKcLV42-83BGKcLV42-84
Sample NameSEC24D-BGKcLV42-83SEC24D-BGKcLV42-84
Pool IDPool-240715Pool-240715
LocalServer_folderset73set73
total_reads38,664,88438,463,312
total_aligned_reads32,787,99032,117,348
unique_aligned_reads30,205,08829,662,175
percent_uniqueAligned0.781200.77118
correlation_replicates0.9952960.995296
spikein_reads3,218514
percent_spikeins0.000080.00001
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID32053206




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database