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RBM10 RNA-Seq in K562



RBM10      CRISPR in K562      Control:

General Information
RBPRBM10
Cell_LineK562
MethodCRISPR
Exp_NameRBM10-BGKcLV42-K562
ENCODE_series_ID
Batch_IDBGKcLV42
Pool IDPool-240715
Local_Set_Nameset73
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1RBM10-BGKcLV42-79
Rep2RBM10-BGKcLV42-80
CN1NT-BGKcLV42-1
CN2NT-BGKcLV42-2
Rep1_qPCR43.1
Rep2_qPCR28.7
Rep1_WBneed discussion
Rep2_WB
Antibody Cat#30774S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1557




Experiment Information (Status: Sequenced)
BGKcLV42-79BGKcLV42-79BGKcLV42-80BGKcLV42-80
idx0000
TRCN#_or_BGC#BGC#0001179BGC#0001179BGC#0001179BGC#0001179
shRNA_or_gRNA_sequenceAGTCCATGTCCCGGTAGTCGAGTCCATGTCCCGGTAGTCGAGTCCATGTCCCGGTAGTCGAGTCCATGTCCCGGTAGTCG
PAMTGGTGGTGGTGG
NameRBM10_95RBM10_95RBM10_95RBM10_95
Sample_IDBGKcLV42-79BGKcLV42-79BGKcLV42-80BGKcLV42-80
transduction_Date4/23/244/23/244/23/244/23/24
daysD6D6D6D6
RBP_nameRBM10RBM10RBM10RBM10
qPCR_result43.143.128.728.7
Ave_qPCR35.935.9
RT-qPCR_primer-Fctttgccttcatccagctctctttgccttcatccagctct
RT-qPCR_primer-Rcccttggcaaactcaacattcccttggcaaactcaacatt
protein_conc2686268626702670
WB_resultneed discussion 58.462.6949
Ave_WB
WB_DONE_date5/10/247/23/24
MW110,135kd110,135kd
IP
antibody_Cat#30774S47729Slot # 1lot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM0000
Rep2_TPM0000
ActionReadyReady
Library_start_date
repeat_library
Notecheck result befor releasecheck result befor release
ID5843584558445846




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RBM10Product_ID: 47729S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: RBM10-human		 CST_47729S_1_RBM10.png<br>Caption: IP-WB analysis of 47729S whole cell lysate using the RBM10 specific antibody, 47729S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RBM10-specific antibody, 47729S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 RBM10-K562-CRISPR-47729S.png<br>Caption: Western blot following CRISPR against RBM10 in K562 whole cell lysate using RBM10 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RBM10. RBM10 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
RBM10Product_ID: 30774S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: RBM10-human		 CST_30774S_1_RBM10.png<br>Caption: IP-WB analysis of 30774S whole cell lysate using the RBM10 specific antibody, 30774S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RBM10-specific antibody, 30774S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 RBM10-K562-CRISPR-30774S.png<br>Caption: Western blot following CRISPR against RBM10 in K562 whole cell lysate using RBM10 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RBM10. RBM10 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV42-79BGKcLV42-80
Sample_IDBGKcLV42-79BGKcLV42-80
Sample Name
Sample NameRBM10-BGKcLV42-79RBM10-BGKcLV42-80
RBPRBM10RBM10
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1310813109




Library-Prep Information
BGKcLV42-79BGKcLV42-80
Sample #6566
Sample NameRBM10-BGKcLV42-79RBM10-BGKcLV42-80
Sample_Name_Alias
Index Well PositionA09B09
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-06-212024-06-21
Lib_IDLib-240621Lib-240621
Tecan_Location
Tecan
Tecan_date
Size_bp324303
Peak_Molarity93.6097.90
libSampleQC_DNA_WellDNA_Library_set73/set1/A9.pngDNA_Library_set73/set1/B9.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set73/set1/A9.pngRNA_Library_set73/set1/B9.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-152024-07-15
Sample_IDBGKcLV42-79BGKcLV42-80
RBPRBM10RBM10
Batch_IDBGKcLV42BGKcLV42
WB_result0.0000.000
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID43344335




Sequencing Information
BGKcLV42-79BGKcLV42-80
Sample_IDBGKcLV42-79BGKcLV42-80
Sample NameRBM10-BGKcLV42-79RBM10-BGKcLV42-80
Pool IDPool-240715Pool-240715
LocalServer_folderset73set73
total_reads62,686,80736,394,250
total_aligned_reads58,515,18132,201,235
unique_aligned_reads54,253,44629,764,773
percent_uniqueAligned0.865470.81784
correlation_replicates0.9756900.975690
spikein_reads5,790792
percent_spikeins0.000090.00002
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID32013202




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database