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ASS1 RNA-Seq in K562



ASS1      CRISPR in K562      Control:

General Information
RBPASS1
Cell_LineK562
MethodCRISPR
Exp_NameASS1-BGKcLV42-K562
ENCODE_series_ID
Batch_IDBGKcLV42
Pool IDPool-240715
Local_Set_Nameset73
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1ASS1-BGKcLV42-7
Rep2ASS1-BGKcLV42-8
CN1NT-BGKcLV42-1
CN2NT-BGKcLV42-2
Rep1_qPCR63.9
Rep2_qPCR64.7
Rep1_WB71.9
Rep2_WB76.4
Antibody Cat#70720S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1527




Experiment Information (Status: Sequenced)
BGKcLV42-7BGKcLV42-8
idx00
TRCN#_or_BGC#BGC#0001110BGC#0001110
shRNA_or_gRNA_sequenceCTTCCCGTAGGTGTTCATTGCTTCCCGTAGGTGTTCATTG
PAMAGGAGG
NameASS1_92ASS1_92
Sample_IDBGKcLV42-7BGKcLV42-8
transduction_Date4/23/244/23/24
daysD6D6
RBP_nameASS1ASS1
qPCR_result63.964.7
Ave_qPCR64.3
RT-qPCR_primer-Fgtggttctggcctacagtgg
RT-qPCR_primer-Rggcttcctcgaagtcttcct
protein_conc33913041
WB_result71.976.4
Ave_WB74.1
WB_DONE_date5/2/24,5/6/24
MW47kd
IP
antibody_Cat#70720Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID57595760




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
ASS1Product_ID: 70720S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: ASS1-human		 CST_70720S_1_ASS1.png<br>Caption: IP-WB analysis of 70720S whole cell lysate using the ASS1 specific antibody, 70720S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the ASS1-specific antibody, 70720S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 ASS1-K562-CRISPR-70720S.png<br>Caption: Western blot following CRISPR against ASS1 in K562 whole cell lysate using ASS1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ASS1. ASS1 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.




Experiment Status
BGKcLV42-7BGKcLV42-8
Sample_IDBGKcLV42-7BGKcLV42-8
Sample Name
Sample NameASS1-BGKcLV42-7ASS1-BGKcLV42-8
RBPASS1ASS1
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1304813049




Library-Prep Information
BGKcLV42-7BGKcLV42-8
Sample #56
Sample NameASS1-BGKcLV42-7ASS1-BGKcLV42-8
Sample_Name_Alias
Index Well PositionE01F01
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-06-212024-06-21
Lib_IDLib-240621Lib-240621
Tecan_Location
Tecan
Tecan_date
Size_bp306303
Peak_Molarity73.2080.20
libSampleQC_DNA_WellDNA_Library_set73/set1/E1.pngDNA_Library_set73/set1/F1.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set73/set1/E1.pngRNA_Library_set73/set1/F1.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-152024-07-15
Sample_IDBGKcLV42-7BGKcLV42-8
RBPASS1ASS1
Batch_IDBGKcLV42BGKcLV42
WB_result71.90076.400
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID42744275




Sequencing Information
BGKcLV42-7BGKcLV42-8
Sample_IDBGKcLV42-7BGKcLV42-8
Sample NameASS1-BGKcLV42-7ASS1-BGKcLV42-8
Pool IDPool-240715Pool-240715
LocalServer_folderset73set73
total_reads54,113,08944,928,148
total_aligned_reads40,374,95838,376,941
unique_aligned_reads37,217,73335,445,851
percent_uniqueAligned0.687780.78895
correlation_replicates0.9972520.997252
spikein_reads4,4404,840
percent_spikeins0.000080.00011
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID31413142




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database