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PARK7 RNA-Seq in K562



PARK7      CRISPR in K562      Control:

General Information
RBPPARK7
Cell_LineK562
MethodCRISPR
Exp_NamePARK7-BGKcLV42-K562
ENCODE_series_ID
Batch_IDBGKcLV42
Pool IDPool-240715
Local_Set_Nameset73
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1PARK7-BGKcLV42-63
Rep2PARK7-BGKcLV42-64
CN1NT-BGKcLV42-1
CN2NT-BGKcLV42-2
Rep1_qPCR76.7
Rep2_qPCR68.4
Rep1_WB82.8
Rep2_WB83.0
Antibody Cat#5560S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1551




Experiment Information (Status: Sequenced)
BGKcLV42-63BGKcLV42-63BGKcLV42-64BGKcLV42-64
idx0000
TRCN#_or_BGC#BGC#0001165BGC#0001165BGC#0001165BGC#0001165
shRNA_or_gRNA_sequenceCACGTCACCTGCACAGATGGCACGTCACCTGCACAGATGGCACGTCACCTGCACAGATGGCACGTCACCTGCACAGATGG
PAMCGGCGGCGGCGG
NamePARK7_71PARK7_71PARK7_71PARK7_71
Sample_IDBGKcLV42-63BGKcLV42-63BGKcLV42-64BGKcLV42-64
transduction_Date4/23/244/23/244/23/244/23/24
daysD6D6D6D6
RBP_namePARK7PARK7PARK7PARK7
qPCR_result76.776.768.468.4
Ave_qPCR72.672.6
RT-qPCR_primer-Ftggtggttctaccaggaggttggtggttctaccaggaggt
RT-qPCR_primer-Rggttttcctgctccttcagtggttttcctgctccttcagt
protein_conc2747274726032603
WB_result82.868.283.068.0284
Ave_WB82.968.1
WB_DONE_date5/8/24, 5/23/246/245/24
MW22kd22kd
IP
antibody_Cat#5560S5933Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM0000
Rep2_TPM0000
ActionReadyReady
Library_start_date
repeat_library
Note
ID5823582558245826




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
PARK7Product_ID: 5560S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: PARK7-human		 PARK7-HEPG2-CRISPR-5560S.png<br>Caption: Western blot following CRISPR against PARK7 in HepG2 whole cell lysate using PARK7 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against PARK7. PARK7 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_5560S_1_PARK7.png<br>Caption: IP-WB analysis of 5560S whole cell lysate using the PARK7 specific antibody, 5560S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the PARK7-specific antibody, 5560S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 PARK7-K562-CRISPR-5560S.png<br>Caption: Western blot following CRISPR against PARK7 in K562 whole cell lysate using PARK7 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against PARK7. PARK7 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
PARK7Product_ID: 5933S
Lot_ID: 1
Source: Cell Signaling
Target Name: PARK7-human		 PARK7-K562-CRISPR-5933S.png<br>Caption: Western blot following CRISPR against PARK7 in K562 whole cell lysate using PARK7 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against PARK7. PARK7 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV42-63BGKcLV42-64
Sample_IDBGKcLV42-63BGKcLV42-64
Sample Name
Sample NamePARK7-BGKcLV42-63PARK7-BGKcLV42-64
RBPPARK7PARK7
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1309613097




Library-Prep Information
BGKcLV42-63BGKcLV42-64
Sample #5354
Sample NamePARK7-BGKcLV42-63PARK7-BGKcLV42-64
Sample_Name_Alias
Index Well PositionE07F07
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-06-212024-06-21
Lib_IDLib-240621Lib-240621
Tecan_Location
Tecan
Tecan_date
Size_bp300297
Peak_Molarity96.2086.90
libSampleQC_DNA_WellDNA_Library_set73/set1/E7.pngDNA_Library_set73/set1/F7.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set73/set1/E7.pngRNA_Library_set73/set1/F7.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-152024-07-15
Sample_IDBGKcLV42-63BGKcLV42-64
RBPPARK7PARK7
Batch_IDBGKcLV42BGKcLV42
WB_result82.80083.000
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID43224323




Sequencing Information
BGKcLV42-63BGKcLV42-64
Sample_IDBGKcLV42-63BGKcLV42-64
Sample NamePARK7-BGKcLV42-63PARK7-BGKcLV42-64
Pool IDPool-240715Pool-240715
LocalServer_folderset73set73
total_reads43,542,96845,277,745
total_aligned_reads38,134,29440,288,475
unique_aligned_reads35,171,96237,200,895
percent_uniqueAligned0.807750.82162
correlation_replicates0.9992320.999232
spikein_reads1,900478
percent_spikeins0.000040.00001
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID31893190




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database