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NOP2 RNA-Seq in K562



NOP2      CRISPR in K562      Control:

General Information
RBPNOP2
Cell_LineK562
MethodCRISPR
Exp_NameNOP2-BGKcLV42-K562
ENCODE_series_ID
Batch_IDBGKcLV42
Pool IDPool-240715
Local_Set_Nameset73
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1NOP2-BGKcLV42-59
Rep2NOP2-BGKcLV42-60
CN1NT-BGKcLV42-1
CN2NT-BGKcLV42-2
Rep1_qPCR0.0
Rep2_qPCR0.0
Rep1_WB89.7
Rep2_WB89.1
Antibody Cat#90709S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1549




Experiment Information (Status: Sequenced)
BGKcLV42-59BGKcLV42-60
idx00
TRCN#_or_BGC#BGC#0001161BGC#0001161
shRNA_or_gRNA_sequenceACCCTACGAAGGAGAAGCGGACCCTACGAAGGAGAAGCGG
PAMGGGGGG
NameNOP2_88NOP2_88
Sample_IDBGKcLV42-59BGKcLV42-60
transduction_Date4/23/244/23/24
daysD6D6
RBP_nameNOP2NOP2
qPCR_result0.00.0
Ave_qPCR0.0
RT-qPCR_primer-Fgagcggctcaagagtgttgt
RT-qPCR_primer-Rcatccagcagtactcggtca
protein_conc667773
WB_result89.789.1
Ave_WB89.4
WB_DONE_date4/30/24
MW110kd
IP
antibody_Cat#90709Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID58195820




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
NOP2Product_ID: 90709S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: NOP2-human		 NOP2-HEPG2-CRISPR-90709S.png<br>Caption: Western blot following CRISPR against NOP2 in HepG2 whole cell lysate using NOP2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against NOP2. NOP2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_90709S_1_NOP2.png<br>Caption: IP-WB analysis of 90709S whole cell lysate using the NOP2 specific antibody, 90709S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the NOP2-specific antibody, 90709S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 NOP2-K562-CRISPR-90709S.png<br>Caption: Western blot following CRISPR against NOP2 in K562 whole cell lysate using NOP2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against NOP2. NOP2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV42-59BGKcLV42-60
Sample_IDBGKcLV42-59BGKcLV42-60
Sample Name
Sample NameNOP2-BGKcLV42-59NOP2-BGKcLV42-60
RBPNOP2NOP2
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1309213093




Library-Prep Information
BGKcLV42-59BGKcLV42-60
Sample #4950
Sample NameNOP2-BGKcLV42-59NOP2-BGKcLV42-60
Sample_Name_Alias
Index Well PositionA07B07
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-06-212024-06-21
Lib_IDLib-240621Lib-240621
Tecan_Location
Tecan
Tecan_date
Size_bp309306
Peak_Molarity126.0099.80
libSampleQC_DNA_WellDNA_Library_set73/set1/A7.pngDNA_Library_set73/set1/B7.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set73/set1/A7.pngRNA_Library_set73/set1/B7.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-152024-07-15
Sample_IDBGKcLV42-59BGKcLV42-60
RBPNOP2NOP2
Batch_IDBGKcLV42BGKcLV42
WB_result89.70089.100
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID43184319




Sequencing Information
BGKcLV42-59BGKcLV42-60
Sample_IDBGKcLV42-59BGKcLV42-60
Sample NameNOP2-BGKcLV42-59NOP2-BGKcLV42-60
Pool IDPool-240715Pool-240715
LocalServer_folderset73set73
total_reads64,054,46843,552,092
total_aligned_reads59,643,07539,154,567
unique_aligned_reads54,778,85135,920,062
percent_uniqueAligned0.855190.82476
correlation_replicates0.9969340.996934
spikein_reads13,8361,586
percent_spikeins0.000220.00004
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID31853186




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database