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NELFCD RNA-Seq in K562



NELFCD      CRISPR in K562      Control:

General Information
RBPNELFCD
Cell_LineK562
MethodCRISPR
Exp_NameNELFCD-BGKcLV42-K562
ENCODE_series_ID
Batch_IDBGKcLV42
Pool IDPool-240715
Local_Set_Nameset73
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1NELFCD-BGKcLV42-57
Rep2NELFCD-BGKcLV42-58
CN1NT-BGKcLV42-1
CN2NT-BGKcLV42-2
Rep1_qPCR50.2
Rep2_qPCR49.9
Rep1_WB74.4
Rep2_WB73.4
Antibody Cat#12265S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1548




Experiment Information (Status: Sequenced)
BGKcLV42-57BGKcLV42-58
idx00
TRCN#_or_BGC#BGC#0001159BGC#0001159
shRNA_or_gRNA_sequenceACTACGGGAGCGCGGCCGAGACTACGGGAGCGCGGCCGAG
PAMTGGTGG
NameNELFCD_92NELFCD_92
Sample_IDBGKcLV42-57BGKcLV42-58
transduction_Date4/23/244/23/24
daysD6D6
RBP_nameNELFCDNELFCD
qPCR_result50.249.9
Ave_qPCR50.0
RT-qPCR_primer-Ftggaaccctccatcttcaac
RT-qPCR_primer-Rccacagcggtgtagttttca
protein_conc28032893
WB_result74.473.4
Ave_WB73.9
WB_DONE_date5/8/24
MW66kd
IP
antibody_Cat#12265Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID58175818




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
NELFCDProduct_ID: 12265S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: NELFCD-human		 CST_12265S_1_NELFCD.png<br>Caption: IP-WB analysis of 12265S whole cell lysate using the NELFCD specific antibody, 12265S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the NELFCD-specific antibody, 12265S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 NELFCD-K562-CRISPR-12265S.png<br>Caption: Western blot following CRISPR against NELFCD in K562 whole cell lysate using NELFCD specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against NELFCD. NELFCD protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV42-57BGKcLV42-58
Sample_IDBGKcLV42-57BGKcLV42-58
Sample Name
Sample NameNELFCD-BGKcLV42-57NELFCD-BGKcLV42-58
RBPNELFCDNELFCD
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1309013091




Library-Prep Information
BGKcLV42-57BGKcLV42-58
Sample #4748
Sample NameNELFCD-BGKcLV42-57NELFCD-BGKcLV42-58
Sample_Name_Alias
Index Well PositionG06H06
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-06-212024-06-21
Lib_IDLib-240621Lib-240621
Tecan_Location
Tecan
Tecan_date
Size_bp292283
Peak_Molarity91.0071.20
libSampleQC_DNA_WellDNA_Library_set73/set1/G6.pngDNA_Library_set73/set1/H6.png
RIN10.09.9
libSample_RNA_WellRNA_Library_set73/set1/G6.pngRNA_Library_set73/set1/H6.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-152024-07-15
Sample_IDBGKcLV42-57BGKcLV42-58
RBPNELFCDNELFCD
Batch_IDBGKcLV42BGKcLV42
WB_result74.40073.400
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID43164317




Sequencing Information
BGKcLV42-57BGKcLV42-58
Sample_IDBGKcLV42-57BGKcLV42-58
Sample NameNELFCD-BGKcLV42-57NELFCD-BGKcLV42-58
Pool IDPool-240715Pool-240715
LocalServer_folderset73set73
total_reads57,085,86954,786,964
total_aligned_reads53,196,45050,470,278
unique_aligned_reads49,260,50446,686,416
percent_uniqueAligned0.862920.85214
correlation_replicates0.9986760.998676
spikein_reads6,4487,224
percent_spikeins0.000110.00013
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID31833184




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database