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NAA10 RNA-Seq in K562



NAA10      CRISPR in K562      Control:

General Information
RBPNAA10
Cell_LineK562
MethodCRISPR
Exp_NameNAA10-BGKcLV42-K562
ENCODE_series_ID
Batch_IDBGKcLV42
Pool IDPool-240715
Local_Set_Nameset73
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1NAA10-BGKcLV42-55
Rep2NAA10-BGKcLV42-56
CN1NT-BGKcLV42-1
CN2NT-BGKcLV42-2
Rep1_qPCR76.1
Rep2_qPCR77.7
Rep1_WB79.6
Rep2_WB79.4
Antibody Cat#13357S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1547




Experiment Information (Status: Sequenced)
BGKcLV42-55BGKcLV42-56
idx00
TRCN#_or_BGC#BGC#0001156BGC#0001156
shRNA_or_gRNA_sequenceACATTGCTGAGGACGAGAATACATTGCTGAGGACGAGAAT
PAMGGGGGG
NameNAA10_87NAA10_87
Sample_IDBGKcLV42-55BGKcLV42-56
transduction_Date4/23/244/23/24
daysD6D6
RBP_nameNAA10NAA10
qPCR_result76.177.7
Ave_qPCR76.9
RT-qPCR_primer-Fttctaccatggcctttcctg
RT-qPCR_primer-Rccaggacataccccacaatc
protein_conc28102612
WB_result79.679.4
Ave_WB79.5
WB_DONE_date4/30/24
MW28kd
IP
antibody_Cat#13357Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID58155816




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
NAA10Product_ID: 13357S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: NAA10-human		 NAA10-HEPG2-CRISPR-13357S.png<br>Caption: Western blot following CRISPR against NAA10 in HepG2 whole cell lysate using NAA10 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against NAA10. NAA10 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_13357S_1_NAA10.png<br>Caption: IP-WB analysis of 13357S whole cell lysate using the NAA10 specific antibody, 13357S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the NAA10-specific antibody, 13357S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 NAA10-K562-CRISPR-13357S.png<br>Caption: Western blot following CRISPR against NAA10 in K562 whole cell lysate using NAA10 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against NAA10. NAA10 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV42-55BGKcLV42-56
Sample_IDBGKcLV42-55BGKcLV42-56
Sample Name
Sample NameNAA10-BGKcLV42-55NAA10-BGKcLV42-56
RBPNAA10NAA10
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1308813089




Library-Prep Information
BGKcLV42-55BGKcLV42-56
Sample #4546
Sample NameNAA10-BGKcLV42-55NAA10-BGKcLV42-56
Sample_Name_Alias
Index Well PositionE06F06
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-06-212024-06-21
Lib_IDLib-240621Lib-240621
Tecan_Location
Tecan
Tecan_date
Size_bp293298
Peak_Molarity112.00104.00
libSampleQC_DNA_WellDNA_Library_set73/set1/E6.pngDNA_Library_set73/set1/F6.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set73/set1/E6.pngRNA_Library_set73/set1/F6.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-152024-07-15
Sample_IDBGKcLV42-55BGKcLV42-56
RBPNAA10NAA10
Batch_IDBGKcLV42BGKcLV42
WB_result79.60079.400
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID43144315




Sequencing Information
BGKcLV42-55BGKcLV42-56
Sample_IDBGKcLV42-55BGKcLV42-56
Sample NameNAA10-BGKcLV42-55NAA10-BGKcLV42-56
Pool IDPool-240715Pool-240715
LocalServer_folderset73set73
total_reads45,689,32039,182,311
total_aligned_reads41,078,48036,461,215
unique_aligned_reads37,952,72233,643,210
percent_uniqueAligned0.830670.85863
correlation_replicates0.9980860.998086
spikein_reads2,750644
percent_spikeins0.000060.00002
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID31813182




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database