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MAPK1 RNA-Seq in K562



MAPK1      CRISPR in K562      Control:

General Information
RBPMAPK1
Cell_LineK562
MethodCRISPR
Exp_NameMAPK1-BGKcLV42-K562
ENCODE_series_ID
Batch_IDBGKcLV42
Pool IDPool-240715
Local_Set_Nameset73
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1MAPK1-BGKcLV42-49
Rep2MAPK1-BGKcLV42-50
CN1NT-BGKcLV42-1
CN2NT-BGKcLV42-2
Rep1_qPCR77.0
Rep2_qPCR70.0
Rep1_WB61.8
Rep2_WB64.1
Antibody Cat#4695S
Antibody Lot#lot # 28
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1546




Experiment Information (Status: Sequenced)
BGKcLV42-49BGKcLV42-50
idx00
TRCN#_or_BGC#BGC#0001153BGC#0001153
shRNA_or_gRNA_sequenceTGGAGCCCTGTACCAACGTGTGGAGCCCTGTACCAACGTG
PAMTGGTGG
NameMAPK1_91MAPK1_91
Sample_IDBGKcLV42-49BGKcLV42-50
transduction_Date4/23/244/23/24
daysD6D6
RBP_nameMAPK1MAPK1
qPCR_result77.070.0
Ave_qPCR73.5
RT-qPCR_primer-Fgtgacctcaagccttccaac
RT-qPCR_primer-Rcctgtgtgatcatggtctgg
protein_conc25522595
WB_result61.864.1
Ave_WB63.0
WB_DONE_date5/7/2002
MW42,44kd
IP
antibody_Cat#4695Slot # 28
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID58095810




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
MAPK1Product_ID: 4695S
Lot_ID: 28
Source: Cell Signaling Technology
Target Name: MAPK1-human		 CST_4695S_28_MAPK1.png<br>Caption: IP-WB analysis of 4695S whole cell lysate using the MAPK specific antibody, 4695S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the MAPK-specific antibody, 4695S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 MAPK1-K562-CRISPR-4695S.png<br>Caption: Western blot following CRISPR against MAPK1 in K562 whole cell lysate using MAPK1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against MAPK1. MAPK1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV42-49BGKcLV42-50
Sample_IDBGKcLV42-49BGKcLV42-50
Sample Name
Sample NameMAPK1-BGKcLV42-49MAPK1-BGKcLV42-50
RBPMAPK1MAPK1
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1308613087




Library-Prep Information
BGKcLV42-49BGKcLV42-50
Sample #4344
Sample NameMAPK1-BGKcLV42-49MAPK1-BGKcLV42-50
Sample_Name_Alias
Index Well PositionC06D06
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-06-212024-06-21
Lib_IDLib-240621Lib-240621
Tecan_Location
Tecan
Tecan_date
Size_bp305304
Peak_Molarity111.0095.40
libSampleQC_DNA_WellDNA_Library_set73/set1/C6.pngDNA_Library_set73/set1/D6.png
RIN9.910.0
libSample_RNA_WellRNA_Library_set73/set1/C6.pngRNA_Library_set73/set1/D6.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-152024-07-15
Sample_IDBGKcLV42-49BGKcLV42-50
RBPMAPK1MAPK1
Batch_IDBGKcLV42BGKcLV42
WB_result61.80064.100
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID43124313




Sequencing Information
BGKcLV42-49BGKcLV42-50
Sample_IDBGKcLV42-49BGKcLV42-50
Sample NameMAPK1-BGKcLV42-49MAPK1-BGKcLV42-50
Pool IDPool-240715Pool-240715
LocalServer_folderset73set73
total_reads34,426,57738,890,956
total_aligned_reads30,030,78735,837,899
unique_aligned_reads27,630,15532,990,723
percent_uniqueAligned0.802580.84829
correlation_replicates0.9972590.997259
spikein_reads3821,026
percent_spikeins0.000010.00003
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID31793180




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database