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LARP1 RNA-Seq in K562



LARP1      CRISPR in K562      Control:

General Information
RBPLARP1
Cell_LineK562
MethodCRISPR
Exp_NameLARP1-BGKcLV42-K562
ENCODE_series_ID
Batch_IDBGKcLV42
Pool IDPool-240715
Local_Set_Nameset73
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1LARP1-BGKcLV42-47
Rep2LARP1-BGKcLV42-48
CN1NT-BGKcLV42-1
CN2NT-BGKcLV42-2
Rep1_qPCR14.2
Rep2_qPCR23.6
Rep1_WB84.5
Rep2_WB86.8
Antibody Cat#70180S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1545




Experiment Information (Status: Sequenced)
BGKcLV42-47BGKcLV42-48
idx00
TRCN#_or_BGC#BGC#0001151BGC#0001151
shRNA_or_gRNA_sequenceGTCTGCCACCTACGTGCCCGGTCTGCCACCTACGTGCCCG
PAMTGGTGG
NameLARP1_92LARP1_92
Sample_IDBGKcLV42-47BGKcLV42-48
transduction_Date4/23/244/23/24
daysD6D6
RBP_nameLARP1LARP1
qPCR_result14.223.6
Ave_qPCR18.9
RT-qPCR_primer-FAACTGCAAGAATACCTCGGC
RT-qPCR_primer-RCACTGAGTGTCGCTTGTGGT
protein_conc25243333
WB_result84.586.8
Ave_WB85.6
WB_DONE_date5/7/2002
MW150kd
IP
antibody_Cat#70180Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID58075808




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
LARP1Product_ID: 70180S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: LARP1-human		 LARP1-HEPG2-CRISPR-70180S.png<br>Caption: Western blot following CRISPR against LARP1 in HepG2 whole cell lysate using LARP1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against LARP1. LARP1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_70180S_1_LARP1.png<br>Caption: IP-WB analysis of 70180S whole cell lysate using the LARP1 specific antibody, 70180S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the LARP1-specific antibody, 70180S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 LARP1-K562-CRISPR-70180S.png<br>Caption: Western blot following CRISPR against LARP1 in K562 whole cell lysate using LARP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against LARP1. LARP1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV42-47BGKcLV42-48
Sample_IDBGKcLV42-47BGKcLV42-48
Sample Name
Sample NameLARP1-BGKcLV42-47LARP1-BGKcLV42-48
RBPLARP1LARP1
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1308413085




Library-Prep Information
BGKcLV42-47BGKcLV42-48
Sample #4142
Sample NameLARP1-BGKcLV42-47LARP1-BGKcLV42-48
Sample_Name_Alias
Index Well PositionA06B06
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-06-212024-06-21
Lib_IDLib-240621Lib-240621
Tecan_Location
Tecan
Tecan_date
Size_bp300298
Peak_Molarity113.0090.00
libSampleQC_DNA_WellDNA_Library_set73/set1/A6.pngDNA_Library_set73/set1/B6.png
RIN9.99.9
libSample_RNA_WellRNA_Library_set73/set1/A6.pngRNA_Library_set73/set1/B6.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-152024-07-15
Sample_IDBGKcLV42-47BGKcLV42-48
RBPLARP1LARP1
Batch_IDBGKcLV42BGKcLV42
WB_result84.50086.800
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID43104311




Sequencing Information
BGKcLV42-47BGKcLV42-48
Sample_IDBGKcLV42-47BGKcLV42-48
Sample NameLARP1-BGKcLV42-47LARP1-BGKcLV42-48
Pool IDPool-240715Pool-240715
LocalServer_folderset73set73
total_reads44,614,74650,468,966
total_aligned_reads42,506,68841,938,656
unique_aligned_reads39,465,28438,612,192
percent_uniqueAligned0.884580.76507
correlation_replicates0.9951530.995153
spikein_reads6,0002,004
percent_spikeins0.000130.00004
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID31773178




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database