Notice: Undefined offset: 1 in /website/encore_alpha/gene/index.php on line 599
KARS RNA-Seq in K562



KARS      CRISPR in K562      Control:

General Information
RBPKARS
Cell_LineK562
MethodCRISPR
Exp_NameKARS-BGKcLV42-K562
ENCODE_series_ID
Batch_IDBGKcLV42
Pool IDPool-240715
Local_Set_Nameset73
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1KARS-BGKcLV42-45
Rep2KARS-BGKcLV42-46
CN1NT-BGKcLV42-1
CN2NT-BGKcLV42-2
Rep1_qPCR90.5
Rep2_qPCR90.6
Rep1_WB96.4
Rep2_WB98.1
Antibody Cat#9990S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1544




Experiment Information (Status: Sequenced)
BGKcLV42-45BGKcLV42-46
idx00
TRCN#_or_BGC#BGC#0001148BGC#0001148
shRNA_or_gRNA_sequenceGACGCCAGTTCCGGAATGAGGACGCCAGTTCCGGAATGAG
PAMGGGGGG
NameKARS_91KARS_91
Sample_IDBGKcLV42-45BGKcLV42-46
transduction_Date4/23/244/23/24
daysD6D6
RBP_nameKARSKARS
qPCR_result90.590.6
Ave_qPCR90.6
RT-qPCR_primer-Ftgtggcaaaagctgttgaat
RT-qPCR_primer-Rgccaaagggctcattatctg
protein_conc27723131
WB_result96.498.1
Ave_WB97.3
WB_DONE_date5/6/24
MW75kd
IP
antibody_Cat#9990Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID58055806




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
KARSProduct_ID: 9990S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: KARS-human		 KARS-HEPG2-CRISPR-9990S.png<br>Caption: Western blot following CRISPR against KARS in HepG2 whole cell lysate using KARS specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against KARS. KARS protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_9990S_1_KARS.png<br>Caption: IP-WB analysis of 9990S whole cell lysate using the KARS specific antibody, 9990S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the KARS-specific antibody, 9990S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 KARS-K562-CRISPR-9990S.png<br>Caption: Western blot following CRISPR against KARS in K562 whole cell lysate using KARS specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against KARS. KARS protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV42-45BGKcLV42-46
Sample_IDBGKcLV42-45BGKcLV42-46
Sample Name
Sample NameKARS-BGKcLV42-45KARS-BGKcLV42-46
RBPKARSKARS
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1308213083




Library-Prep Information
BGKcLV42-45BGKcLV42-46
Sample #3940
Sample NameKARS-BGKcLV42-45KARS-BGKcLV42-46
Sample_Name_Alias
Index Well PositionG05H05
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-06-212024-06-21
Lib_IDLib-240621Lib-240621
Tecan_Location
Tecan
Tecan_date
Size_bp288288
Peak_Molarity84.3087.00
libSampleQC_DNA_WellDNA_Library_set73/set1/G5.pngDNA_Library_set73/set1/H5.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set73/set1/G5.pngRNA_Library_set73/set1/H5.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-152024-07-15
Sample_IDBGKcLV42-45BGKcLV42-46
RBPKARSKARS
Batch_IDBGKcLV42BGKcLV42
WB_result96.40098.100
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID43084309




Sequencing Information
BGKcLV42-45BGKcLV42-46
Sample_IDBGKcLV42-45BGKcLV42-46
Sample NameKARS-BGKcLV42-45KARS-BGKcLV42-46
Pool IDPool-240715Pool-240715
LocalServer_folderset73set73
total_reads43,401,16338,540,417
total_aligned_reads38,023,28031,889,779
unique_aligned_reads35,070,17529,452,181
percent_uniqueAligned0.808050.76419
correlation_replicates0.9982250.998225
spikein_reads4,4704,704
percent_spikeins0.000100.00012
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID31753176




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database