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HEXIM1 RNA-Seq in K562



HEXIM1      CRISPR in K562      Control:

General Information
RBPHEXIM1
Cell_LineK562
MethodCRISPR
Exp_NameHEXIM1-BGKcLV42-K562
ENCODE_series_ID
Batch_IDBGKcLV42
Pool IDPool-240715
Local_Set_Nameset73
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1HEXIM1-BGKcLV42-39
Rep2HEXIM1-BGKcLV42-40
CN1NT-BGKcLV42-1
CN2NT-BGKcLV42-2
Rep1_qPCR17.0
Rep2_qPCR2.9
Rep1_WB94.7
Rep2_WB96.0
Antibody Cat#12604S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1542




Experiment Information (Status: Sequenced)
BGKcLV42-39BGKcLV42-40
idx00
TRCN#_or_BGC#BGC#0001142BGC#0001142
shRNA_or_gRNA_sequenceAGAAGTGGAACCGACGCCCGAGAAGTGGAACCGACGCCCG
PAMAGGAGG
NameHEXIM1_95HEXIM1_95
Sample_IDBGKcLV42-39BGKcLV42-40
transduction_Date4/23/244/23/24
daysD6D6
RBP_nameHEXIM1HEXIM1
qPCR_result17.02.9
Ave_qPCR10.0
RT-qPCR_primer-Ftgtccaggaagagctgaacc
RT-qPCR_primer-Rcgattgccacctactgtcct
protein_conc30723212
WB_result94.796.0
Ave_WB95.4
WB_DONE_date5/6/24
MW60kd
IP
antibody_Cat#12604Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID57995800




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
HEXIM1Product_ID: 12604S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: HEXIM1-human		 HEXIM1-HEPG2-CRISPR-12604S.png<br>Caption: Western blot following CRISPR against HEXIM1 in HepG2 whole cell lysate using HEXIM1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against HEXIM1. HEXIM1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_12604S_1_HEXIM1.png<br>Caption: IP-WB analysis of 12604S whole cell lysate using the HEXIM1 specific antibody, 12604S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the HEXIM1-specific antibody, 12604S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 HEXIM1-K562-CRISPR-12604S.png<br>Caption: Western blot following CRISPR against HEXIM1 in K562 whole cell lysate using HEXIM1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against HEXIM1. HEXIM1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV42-39BGKcLV42-40
Sample_IDBGKcLV42-39BGKcLV42-40
Sample Name
Sample NameHEXIM1-BGKcLV42-39HEXIM1-BGKcLV42-40
RBPHEXIM1HEXIM1
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1307813079




Library-Prep Information
BGKcLV42-39BGKcLV42-40
Sample #3536
Sample NameHEXIM1-BGKcLV42-39HEXIM1-BGKcLV42-40
Sample_Name_Alias
Index Well PositionC05D05
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-06-212024-06-21
Lib_IDLib-240621Lib-240621
Tecan_Location
Tecan
Tecan_date
Size_bp296313
Peak_Molarity115.00115.00
libSampleQC_DNA_WellDNA_Library_set73/set1/C5.pngDNA_Library_set73/set1/D5.png
RIN10.09.9
libSample_RNA_WellRNA_Library_set73/set1/C5.pngRNA_Library_set73/set1/D5.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-152024-07-15
Sample_IDBGKcLV42-39BGKcLV42-40
RBPHEXIM1HEXIM1
Batch_IDBGKcLV42BGKcLV42
WB_result94.70096.000
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID43044305




Sequencing Information
BGKcLV42-39BGKcLV42-40
Sample_IDBGKcLV42-39BGKcLV42-40
Sample NameHEXIM1-BGKcLV42-39HEXIM1-BGKcLV42-40
Pool IDPool-240715Pool-240715
LocalServer_folderset73set73
total_reads42,918,96631,623,673
total_aligned_reads37,134,03327,503,021
unique_aligned_reads34,276,91225,336,803
percent_uniqueAligned0.798640.80120
correlation_replicates0.9992220.999222
spikein_reads3901,530
percent_spikeins0.000010.00005
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID31713172




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database