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EIF4H RNA-Seq in K562



EIF4H      CRISPR in K562      Control:

General Information
RBPEIF4H
Cell_LineK562
MethodCRISPR
Exp_NameEIF4H-BGKcLV42-K562
ENCODE_series_ID
Batch_IDBGKcLV42
Pool IDPool-240715
Local_Set_Nameset73
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1EIF4H-BGKcLV42-35
Rep2EIF4H-BGKcLV42-36
CN1NT-BGKcLV42-1
CN2NT-BGKcLV42-2
Rep1_qPCR0.0
Rep2_qPCR9.0
Rep1_WB91.3
Rep2_WB90.5
Antibody Cat#3469S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1540




Experiment Information (Status: Sequenced)
BGKcLV42-35BGKcLV42-36
idx00
TRCN#_or_BGC#BGC#0001137BGC#0001137
shRNA_or_gRNA_sequenceAATCCATGTTGGATCCACGGAATCCATGTTGGATCCACGG
PAMAGGAGG
NameEIF4H_74EIF4H_74
Sample_IDBGKcLV42-35BGKcLV42-36
transduction_Date4/23/244/23/24
daysD6D6
RBP_nameEIF4HEIF4H
qPCR_result0.09.0
Ave_qPCR4.5
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc38593474
WB_result91.390.5
Ave_WB90.9
WB_DONE_date5/2/24
MW25,27kd
IP
antibody_Cat#3469Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID57955796




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EIF4HProduct_ID: 3469S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: EIF4H-human		 EIF4H-HEPG2-CRISPR-3469S.png<br>Caption: Western blot following CRISPR against EIF4H in HepG2 whole cell lysate using EIF4H specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF4H. EIF4H protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_3469S_1_EIF4H.png<br>Caption: IP-WB analysis of 3469S whole cell lysate using the EIF4H specific antibody, 3469S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the EIF4H-specific antibody, 3469S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 EIF4H-K562-CRISPR-3469S.png<br>Caption: Western blot following CRISPR against EIF4H in K562 whole cell lysate using EIF4H specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF4H. EIF4H protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV42-35BGKcLV42-36
Sample_IDBGKcLV42-35BGKcLV42-36
Sample Name
Sample NameEIF4H-BGKcLV42-35EIF4H-BGKcLV42-36
RBPEIF4HEIF4H
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1307413075




Library-Prep Information
BGKcLV42-35BGKcLV42-36
Sample #3132
Sample NameEIF4H-BGKcLV42-35EIF4H-BGKcLV42-36
Sample_Name_Alias
Index Well PositionG04H04
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-06-212024-06-21
Lib_IDLib-240621Lib-240621
Tecan_Location
Tecan
Tecan_date
Size_bp287300
Peak_Molarity84.9092.50
libSampleQC_DNA_WellDNA_Library_set73/set1/G4.pngDNA_Library_set73/set1/H4.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set73/set1/G4.pngRNA_Library_set73/set1/H4.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-152024-07-15
Sample_IDBGKcLV42-35BGKcLV42-36
RBPEIF4HEIF4H
Batch_IDBGKcLV42BGKcLV42
WB_result91.30090.500
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID43004301




Sequencing Information
BGKcLV42-35BGKcLV42-36
Sample_IDBGKcLV42-35BGKcLV42-36
Sample NameEIF4H-BGKcLV42-35EIF4H-BGKcLV42-36
Pool IDPool-240715Pool-240715
LocalServer_folderset73set73
total_reads50,701,90749,352,977
total_aligned_reads47,417,86043,940,279
unique_aligned_reads43,712,95840,438,834
percent_uniqueAligned0.862160.81938
correlation_replicates0.9940710.994071
spikein_reads6,2308,670
percent_spikeins0.000120.00018
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID31673168




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database